Human DEDD Recombinant Protein (RPPB3347)

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SKU:
RPPB3347
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
DEDD
Synonyms:
CASP8IP1
Synonyms:
DEDD1
Synonyms:
DEFT
Synonyms:
FLDED1
Source:
Escherichia Coli
Uniprot:
O75618
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Description

Product Name:Human DEDD Recombinant Protein
Product Code:RPPB3347
Size:10µg
Species:Human
Target:DEDD
Synonyms:CASP8IP1, DEDD1, DEFT, FLDED1, KE05, DEDPro1, Death effector domain-containing testicular molecule.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:DEDD protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAGLKRRASQ VWPEEHGEQE HGLYSLHRMF DIVGTHLTHR DVRVLSFLFV DVIDDHERGL IRNGRDFLLA LERQGRCDES NFRQVLQLLR IITRHDLLPY VTLKRRRAVC PDLVDKYLEE TSIRYVTPRA LSDPEPRPPQ PSKTVPPHYP VVCCPTSGPQ MCSKRPARGR ATLGSQRKRR KSVTPDPKEK QTCDIRLRVR AEYCQHETAL QGNVFSNKQD PLERQFERFN QANTILKSRD LGSIICDIKF SELTYLDAFW RDYINGSLLE ALKGVFITDS LKQAVGHEAI KLLVNVDEED YELGRQKLLR NLMLQALP

Death effector domain-containing protein (DEDD) is a cytoplasmic protein. The cell death activity of DEDD relates to its nuclear localization. DEDD translocates to the nucleus during CD95-mediated apoptosis, there it localizes to nucleoli-like structures, activates caspase-6 and particularly inhibits RNA polymerase I-dependent transcription. DEDD is usually expressed in a variety of tissues, and found in the highest levels in the testis. Overexpression of DEDD was shown to induce weak apoptosis.

DEDD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (1-318a.a) and having a molecular mass of 38.9kDa. DEDD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DEDD: A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleolus

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; nucleolus

Molecular Function:DNA binding; protein binding

NCBI Summary:This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O75618
NCBI GenInfo Identifier:21759089
NCBI Gene ID:9191
NCBI Accession:O75618.1
UniProt Secondary Accession:O75618,O60737, D3DVF5,
UniProt Related Accession:O75618
Molecular Weight:40,418 Da
NCBI Full Name:Death effector domain-containing protein
NCBI Synonym Full Names:death effector domain containing
NCBI Official Symbol:DEDD  
NCBI Official Synonym Symbols:DEFT; KE05; DEDD1; FLDED1; CASP8IP1  
NCBI Protein Information:death effector domain-containing protein
UniProt Protein Name:Death effector domain-containing protein
UniProt Synonym Protein Names:DEDPro1; Death effector domain-containing testicular molecule; FLDED-1
Protein Family:Death effector domain-containing protein
UniProt Gene Name:DEDD  
UniProt Entry Name:DEDD_HUMAN
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