Human CRYBB1 Recombinant Protein (RPPB5197)
- SKU:
- RPPB5197
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- CRYBB1
- Synonyms:
- EC 11741
- RR2M
- Beta-B1 crystallin
- CATCN3
- Source:
- Escherichia Coli
- Uniprot:
- P53674
Description
| Product Name: | Human CRYBB1 Recombinant Protein |
| Product Code: | RPPB5197 |
| Size: | 25µg |
| Species: | Human |
| Target: | CRYBB1 |
| Synonyms: | EC 1.17.4.1, RR2M, Beta-B1 crystallin, CATCN3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The CRYBB1 solution (1mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSQAAKASAS ATVAVNPGPD TKGKGAPPAG TSPSPGTTLA PTTVPITSAK AAELPPGNYR LVVFELENFQ GRRAEFSGEC SNLADRGFDR VRSIIVSAGP WVAFEQSNFR GEMFILEKGE YPRWNTWSSS YRSDRLMSFR PIKMDAQEHK ISLFEGANFK GNTIEIQGDD APSLWVYGFS DRVGSVKVSS GTWVGYQYPG YRGYQYLLEP GDFRHWNEWG AFQPQMQSLR RLRDKQWHLE GSFPVLATEP PKRSHHHHHH |
Crystallins are the main structural proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into 3 fprotein families, ?, Beta, & ? families. Because lens central fiber cells lose their nuclei during development, these crystallins are prepared and then retained throughout life, making them extremely stable proteins. CRYBB1 is a beta basic group member and undergoes extensive cleavage at its N-terminal extension during lens maturation.
CRYBB1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 260 amino acids (1-252 a.a.) and having a molecular mass of 29.1 kDa. The CRYBB1 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CRYBB1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization. The protease responsible for this partial degradation could be calpain II. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 22q12.1 Molecular Function:structural constituent of eye lens Biological Process: visual perception Disease: Cataract 17, Multiple Types |
| NCBI Summary: | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P53674 |
| NCBI GenInfo Identifier: | 1706116 |
| NCBI Gene ID: | 1414 |
| NCBI Accession: | P53674.2 |
| UniProt Secondary Accession: | P53674,Q9WVJ5, |
| UniProt Related Accession: | P53674 |
| Molecular Weight: | 252 |
| NCBI Full Name: | Beta-crystallin B1 |
| NCBI Synonym Full Names: | crystallin, beta B1 |
| NCBI Official Symbol: | CRYBB1 |
| NCBI Official Synonym Symbols: | CATCN3; CTRCT17 |
| NCBI Protein Information: | beta-crystallin B1; beta-B1 crystallin; eye lens structural protein |
| UniProt Protein Name: | Beta-crystallin B1 |
| UniProt Synonym Protein Names: | Beta-B1 crystallin |
| Protein Family: | Beta-crystallin |
| UniProt Gene Name: | CRYBB1 |
| UniProt Entry Name: | CRBB1_HUMAN |
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