The Human CK-18 (KRT18) Cytokeratin 18 ELISA Kit is a reliable and accurate tool for quantifying levels of CK-18 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CK-18, a member of the cytokeratin family, is a key structural protein found in epithelial cells and tissues. Abnormal levels of CK-18 have been associated with various diseases and conditions, including liver diseases, cancer, and inflammatory disorders.
As such, measuring CK-18 levels can provide valuable insights into disease progression, severity, and treatment efficacy.By using the Human CK-18 Cytokeratin 18 ELISA Kit, researchers can gain a better understanding of CK-18 biology and its implications in human health and disease. This kit is a valuable resource for studying CK-18-related pathways, identifying potential biomarkers, and developing novel therapeutic strategies.
Product Name:
Human CK-18/KRT18 (Cytokeratin 18) ELISA Kit
SKU:
HUES02985
Target:
Human CK-18/KRT18 (Cytokeratin 18)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 mIU/mL
Detection range:
6.25-400 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CK-18/KRT18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK-18/KRT18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK-18/KRT18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CK-18/KRT18. You can calculate the concentration of Human CK-18/KRT18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
93-107
94-110
Average (%)
98
99
102
1:4
Range (%)
92-108
81-92
86-100
Average (%)
98
87
93
1:8
Range (%)
88-102
83-95
84-94
Average (%)
94
89
89
1:16
Range (%)
92-107
81-96
83-95
Average (%)
98
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
95-107
100
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.51
61.86
159.46
19.61
63.54
150.53
Standard deviation
1.12
2.5
7.35
1.13
3.18
5.81
C V (%)
6.05
4.04
4.61
5.76
5.0
3.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CK-18/KRT18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
