The CD235 Monoclonal Antibody (FITC Conjugated) is a specialized tool for research involving human CD235, a protein marker found on red blood cells. This antibody, labeled with FITC for fluorescence detection, is specifically designed to detect and analyze CD235 in various biological samples. It is ideal for use in flow cytometry and immunofluorescence applications, allowing for precise identification and quantification of CD235-expressing cells.CD235, also known as glycophorin A, is a key antigen present on the surface of erythrocytes and plays a crucial role in blood group typing and transfusion medicine. The highly specific reactivity of the CD235 Monoclonal Antibody makes it a reliable tool for studying red blood cell biology, hematological disorders, and transfusion compatibility.
Researchers investigating conditions such as anemia, hemolytic diseases, and blood transfusion reactions will benefit from the precise targeting and detection capabilities of this antibody.By providing a targeted approach to studying CD235 expression, this FITC-conjugated monoclonal antibody enhances the accuracy and efficiency of experiments focusing on red blood cell-related research. Its reliable performance and sensitivity make it an indispensable tool for scientists exploring the diverse functions and implications of CD235 in various biological contexts.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P02724 P06028
Gene ID:
2993
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.