The Human Casp-9 (Caspase-9) ELISA Kit is specifically designed for the quantitative detection of caspase-9 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Caspase-9 is a key player in the apoptotic pathway, playing a crucial role in programmed cell death. Dysregulation of caspase-9 activity has been implicated in various diseases, including cancer and neurodegenerative disorders.
This ELISA kit provides researchers with a valuable tool for studying the role of caspase-9 in these conditions and developing potential therapeutic interventions.Overall, the Human Casp-9 ELISA Kit from Assay Genie offers a reliable and convenient solution for quantifying caspase-9 levels, ultimately advancing research in apoptosis-related diseases.
Product Name:
Human CASP 9 (Caspase 9) ELISA Kit
SKU:
HUES01822
Target:
Human CASP 9 (Caspase 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP9. You can calculate the concentration of Human CASP9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
98-113
96-111
Average (%)
96
105
102
1:4
Range (%)
92-102
79-92
88-101
Average (%)
97
85
94
1:8
Range (%)
89-102
80-90
86-99
Average (%)
95
85
91
1:16
Range (%)
92-104
81-91
82-96
Average (%)
98
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.74
10.62
36.84
4.72
10.55
35.76
Standard deviation
0.25
0.54
1.33
0.3
0.48
1.41
C V (%)
5.27
5.08
3.61
6.36
4.55
3.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CASP9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP9 in samples. No significant cross-reactivity or interference between Human CASP9 and analogues was observed.
