The Human Casp-9 (Caspase-9) CLIA Kit is a highly sensitive assay designed for the accurate detection of Caspase-9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior specificity and reliability, ensuring consistent and reproducible results for a variety of research applications.Caspase-9 is a key enzyme involved in apoptosis, playing a critical role in programmed cell death. Dysregulation of Caspase-9 has been implicated in various diseases including cancer, immune disorders, and neurodegenerative conditions.
Therefore, accurate measurement of Caspase-9 levels is essential for understanding disease mechanisms and developing potential therapeutic interventions. The Human Casp-9 CLIA Kit from Assay Genie provides researchers with a powerful tool to investigate the role of Caspase-9 in health and disease. With its high sensitivity and precision, this kit is a valuable asset for researchers studying apoptosis, cell signaling pathways, and potential drug targets.
Product Name:
Human CASP 9 (Caspase 9) CLIA Kit
SKU:
HUES00438
Target:
Human CASP 9 (Caspase 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP9. You can calculate the concentration of Human CASP9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
87-98
86-101
Average (%)
94
93
92
1:4
Range (%)
89-102
89-105
95-110
Average (%)
95
96
101
1:8
Range (%)
87-100
90-104
100-115
Average (%)
92
96
107
1:16
Range (%)
89-103
92-104
94-107
Average (%)
95
99
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
87-102
95
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.11
8.2
0.97
2.31
7.7
Standard deviation
0.09
0.19
0.69
0.12
0.23
0.61
C V (%)
9.28
9.0
8.41
12.37
9.96
7.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP9 in samples. No significant cross-reactivity or interference between Human CASP9 and analogues was observed.
