Human CA8 Recombinant Protein (RPPB1463)

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SKU:
RPPB1463
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
CA8
Synonyms:
Carbonic anhydrase-related protein
Synonyms:
CA-VIII
Synonyms:
CALS
Synonyms:
CAMRQ3
Source:
Escherichia Coli
Uniprot:
P35219
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Description

Product Name:Human CA8 Recombinant Protein
Product Code:RPPB1463
Size:10µg
Species:Human
Target:CA8
Synonyms:Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:CA8 Human protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVEWGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSKSVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEAVGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYWVYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ
Biological Activity:Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Carbonic Anhydrase VIII or CA8 was previously called CA-related protein due to its sequence resemblance to additional recognized carbonic anhydrase genes. Nonetheless CA8 doesn’t have carbonic anhydrase function. This protein keeps bearing a carbonic anhydrase classification because of coherent sequence similarity to additional proteins in carbonic anhydrase family. Mutations in this protein may lead to cerebellar dysequilibrium syndrome type 3 or ataxia mental retardation.

CA8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-290) and having a molecular mass of 35.5kDa.CA8 Humanis fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Does not have a carbonic anhydrase catalytic activity.CautionAlthough it belongs to the alpha-carbonic anhydrase family, Arg-116 is present instead of the conserved His which is a zinc-binding residue. It is therefore expected that this protein lacks carbonic anhydrase activity.
NCBI Summary:The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:P35219
NCBI GenInfo Identifier:461681
NCBI Gene ID:767
NCBI Accession:P35219.3
UniProt Secondary Accession:P35219,Q32MY2, A8K0A5, B3KQZ7,
UniProt Related Accession:P35219
Molecular Weight:33 kDa
NCBI Full Name:Carbonic anhydrase-related protein
NCBI Synonym Full Names:carbonic anhydrase 8
NCBI Official Symbol:CA8  
NCBI Official Synonym Symbols:CALS; CARP; CA-RP; CAMRQ3; CA-VIII  
NCBI Protein Information:carbonic anhydrase-related protein
UniProt Protein Name:Carbonic anhydrase-related protein
UniProt Synonym Protein Names:Carbonic anhydrase VIII; CA-VIII
Protein Family:Carbonic anhydrase-related protein
UniProt Gene Name:CA8  
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