Human CA13 Recombinant Protein (RPPB1467)
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주문- SKU:
- RPPB1467
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- CA13
- Synonyms:
- Carbonic AnhydraseXIII
- Synonyms:
- Carbonate Dehydratase XIII
- Synonyms:
- EC 4211
- Synonyms:
- CA-XIII
- Source:
- Escherichia Coli
- Uniprot:
- Q8N1Q1
Description
| Product Name: | Human CA13 Recombinant Protein |
| Product Code: | RPPB1467 |
| Size: | 10µg |
| Species: | Human |
| Target: | CA13 |
| Synonyms: | Carbonic AnhydraseXIII, Carbonate Dehydratase XIII, EC 4.2.1.1, CA-XIII, Carbonic, Anhydrase 13, CAXIII. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | CA13 proteinsolution (0.5mg/ml) containing Phosphate buffered saline (pH7.4),10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% asdetermined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSPMGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSFNVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAHEPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLESVTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH |
| Biological Activity: | Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C. |
Carbonic Anhydrase XIII, also known as CA13 is a member of thealpha-carbonic anhydrase family, which catalyzes the rapid interconversion ofcarbon dioxide and water to bicarbonate andprotons, a reversible reaction which occurs relatively slowly in the absence ofcatalyst. Furthermore, the active site of nearly allcarbonic anhydrases contains a zinc ion; they have been classified asmetalloenzymes.
CA13 HumanRecombinant produced in E.Coli is a single, non-glycosylated polypeptide chaincontaining 285 amino acids (1-262a.a) and having a molecular mass of 31.8kDa.CA13 is fused to a 23amino acid His-tag at N-terminus & purified by proprietary chromatographictechniques.
| UniProt Protein Function: | CA13: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen Chromosomal Location of Human Ortholog: 8q21.2 Cellular Component: cytosol; myelin sheath Molecular Function:carbonate dehydratase activity; zinc ion binding Biological Process: bicarbonate transport; one-carbon compound metabolic process |
| NCBI Summary: | Carbonic anhydrases (CAs) are a family of zinc metalloenzymes. For background information on the CA family, see MIM 114800.[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q8N1Q1 |
| NCBI GenInfo Identifier: | 38348436 |
| NCBI Gene ID: | 377677 |
| NCBI Accession: | NP_940986.1 |
| UniProt Related Accession: | Q8N1Q1 |
| Molecular Weight: | 29,443 Da |
| NCBI Full Name: | carbonic anhydrase 13 |
| NCBI Synonym Full Names: | carbonic anhydrase XIII |
| NCBI Official Symbol: | CA13 |
| NCBI Official Synonym Symbols: | CAXIII |
| NCBI Protein Information: | carbonic anhydrase 13; CA-XIII; carbonate dehydratase XIII |
| UniProt Protein Name: | Carbonic anhydrase 13 |
| UniProt Synonym Protein Names: | Carbonate dehydratase XIII; Carbonic anhydrase XIII; CA-XIII |
| Protein Family: | Carbonic anhydrase |
| UniProt Gene Name: | CA13 |
| UniProt Entry Name: | CAH13_HUMAN |
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