The Human C3d Complement Fragment 3D ELISA Kit is a powerful tool for the precise measurement of C3d complement fragment levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable asset for a variety of research endeavors.The C3d complement fragment is a key component of the complement system, playing a crucial role in immune responses and inflammatory processes.
Abnormal levels of C3d have been linked to various autoimmune disorders, infectious diseases, and inflammatory conditions, highlighting its significance as a potential biomarker for disease diagnostics and therapeutic interventions.By utilizing the Human C3d Complement Fragment 3D ELISA Kit, researchers can gain valuable insights into the role of C3d in health and disease, paving the way for new discoveries and advancements in the field of immunology and biomedical research.
Product Name:
Human C3d (Complement Fragment 3d) ELISA Kit
SKU:
HUES03450
Target:
Human C3d (Complement Fragment 3d)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3d. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3d and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3d, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3d. You can calculate the concentration of Human C3d in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
86-97
94-109
Average (%)
97
91
100
1:4
Range (%)
86-100
83-95
85-100
Average (%)
92
90
92
1:8
Range (%)
86-102
81-95
88-100
Average (%)
94
87
94
1:16
Range (%)
89-101
85-95
81-94
Average (%)
95
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.04
59.71
228.41
26.44
62.54
241.76
Standard deviation
1.36
2.88
8.31
1.85
2.92
8.73
C V (%)
5.03
4.82
3.64
7.0
4.67
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C3d concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C3d in samples. No significant cross-reactivity or interference between Human C3d and analogues was observed.
