The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
Product Name:
Human BLMH/BLM Hydrolase Recombinant Protein (RPES1872)
Product Code:
RPES1872
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
BH,BMH
Accession:
NP_000377.1
Sequence:
Ser 2-Glu 455
Fusion tag:
N-His
Activity:
Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/µg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human BLMH (NP_000377.1) (Ser 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.
Purity:
> 88 % as determined by reducing SDS-PAGE.
Mol Mass:
53.4 kDa
AP Mol Mass:
53.4 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
