Human BAT1 Recombinant Protein (RPPB1436)

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SKU:
RPPB1436
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
BAT1
Synonyms:
HLA-B associated transcript 1
Synonyms:
UAP56
Synonyms:
D6S81E
Synonyms:
DDX39B
Source:
Escherichia Coli
Uniprot:
Q13838
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Description

Product Name:Human BAT1 Recombinant Protein
Product Code:RPPB1436
Size:10µg
Species:Human
Target:BAT1
Synonyms:HLA-B associated transcript 1, UAP56, D6S81E, DDX39B, ATP-dependent RNA helicase p47, DEAD-box protein UAP56
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:BAT1 protein (0.5mg/ml) solution contains 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl,1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAENDVDNEL LDYEDDEVET AAGGDGAEAP AKKDVKGSYV SIHSSGFRDF LLKPELLRAI VDCGFEHPSE VQHECIPQAI LGMDVLCQAK SGMGKTAVFV LATLQQLEPV TGQVSVLVMC HTRELAFQIS KEYERFSKYM PNVKVAVFFG GLSIKKDEEV LKKNCPHIVV GTPGRILALA RNKSLNLKHI KHFILDECDK MLEQLDMRRD VQEIFRMTPH EKQVMMFSAT LSKEIRPVCR KFMQDPMEIF VDDETKLTLH GLQQYYVKLK DNEKNRKLFD LLDVLEFNQV VIFVKSVQRC IALAQLLVEQ NFPAIAIHRG MPQEERLSRY QQFKDFQRRI LVATNLFGRG MDIERVNIAF NYDMPEDSDT YLHRVARAGR FGTKGLAITF VSDENDAKIL NDVQDRFEVN ISELPDEIDI SSYIEQTR

BAT1 belongs to the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. UAP56 protein is a vital splicing factor which is needed for connecting U2 small nuclear ribonucleoprotein with pre-mRNA, as well as takes a key part in mRNA export from the nucleus to the cytoplasm. Mutations in this protein may be connected to rheumatoid arthritis.

BAT1 Human Recombinant protein produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 448 amino acids (1-428) and having a molecular mass of 51.1kDa. BAT1 is fused to 20 amino acid His Tag at N-terminus and is purified by proprietary chromatographic techniques.

UniProt Protein Function:BAT1: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Belongs to the DEAD box helicase family. DECD subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Spliceosome; EC 3.6.4.13; RNA splicing; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; nucleoplasm; nucleus; spliceosome

Molecular Function:ATP-dependent protein binding; ATP-dependent RNA helicase activity; ATPase activity; protein binding; RNA-dependent ATPase activity; U4 snRNA binding; U6 snRNA binding

Biological Process: intronless viral mRNA export from host nucleus; mRNA 3'-end processing; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; positive regulation of RNA elongation; RNA export from nucleus; RNA secondary structure unwinding; RNA splicing; spliceosome assembly; termination of RNA polymerase II transcription

NCBI Summary:This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene. [provided by RefSeq, Feb 2011]
UniProt Code:Q13838
NCBI GenInfo Identifier:2500529
NCBI Gene ID:7919
NCBI Accession:Q13838.1
UniProt Secondary Accession:Q13838,O43496, Q0EFA1, Q2L6F9, Q53GL9, Q5RJ64, Q5RJ66 Q5ST94, Q5STB4, Q5STB5, Q5STB7, B0S8C0,
UniProt Related Accession:Q13838
Molecular Weight:50,679 Da
NCBI Full Name:Spliceosome RNA helicase DDX39B
NCBI Synonym Full Names:DEAD-box helicase 39B
NCBI Official Symbol:DDX39B  
NCBI Official Synonym Symbols:BAT1; UAP56; D6S81E  
NCBI Protein Information:spliceosome RNA helicase DDX39B
UniProt Protein Name:Spliceosome RNA helicase DDX39B
UniProt Synonym Protein Names:56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein
Protein Family:Spliceosome RNA helicase
UniProt Gene Name:DDX39B  
UniProt Entry Name:DX39B_HUMAN
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