Human AURKA Recombinant Protein (RPPB2441)

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SKU:
RPPB2441
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
AURKA
Synonyms:
Serine/threonine-protein kinase 6
Synonyms:
Aurora kinase A
Synonyms:
Serine/threonine kinase 15
Synonyms:
Aurora/IPL1-related kinase 1
Source:
Escherichia Coli
Uniprot:
O14965
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Description

Product Name:Human AURKA Recombinant Protein
Product Code:RPPB2441
Size:10µg
Species:Human
Target:AURKA
Synonyms:Serine/threonine-protein kinase 6, Aurora kinase A, Serine/threonine kinase 15, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Aurora-A, Aurora-related kinase 1, hARK1, AURKA, AIK, ARK1, AURA, BTAK, STK15, STK6, STK7, STK15, AURORA2, MGC34538.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The AURKA solution containing 20mM Tris-HCl buffer (pH 8.0), 0.5mM DTT, 100mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF and 20% glycerol.
Stability:AURKA although stable at 4°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILALKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKRISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS

AURKA (Aurora Kinase A) belongs to the mitotic serine/threonine kinases family. AURKA is a cell cycle-regulated kinase which may be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. AURKA is found at the centrosome in interphase cells and at the spindle poles in mitosis. Since the AURKA expression is cell-cycle regulated, it is low in G1/S, it accumulates during G2/M, and it decreases rapidly after. AURKA is involved in important processes during mitosis and meiosis whose proper function is essential for healthy cell proliferation. In addition, AURKA plays an essential role in tumourigenesis and is overexpressed in various types of cancers. AURKA is strongly expressed in the testis, colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines and weakly in skeletal muscle, thymus and spleen. AURKA interacts with its substrates BORA and ARHGEF2, as well as with TACC1 and CPEB1.Defects in the AURKA gene cause numerical centrosome aberrations including aneuploidy. AURKA overexpression has been linked to chromosomal instability in colorectal cancer. AURKA expression may have a prognostic significance in ovarian carcinoma.

AURKA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 423 amino acids (1-403) and having a molecular mass of 47.9kDa. AURKA is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:AurA: a member of the AUR family of kinases. A cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines. Regulated by phosphorylation-dependent proteasomal degradation. Localized to mitotic centrosomes and spindle microtubules and is required for centrosome maturation. Loss of Aurora A leads to defective mitotic spindles and gross errors in chromosome segregation. Required for centrosome duplication and chromosome segregation. Overexpression in culture drives transformation and aneuploidy, and negatively regulates p53. Amplified or overexpressed in many tumors or cell lines. Found as a skin tumor susceptibility gene in mouse, and a human SNP in a degradation domain is weakly cancer-associated and undergoes allele-specific amplification. Inhibitors: VX-680, ZM447439, Hesperadin, SNS-595.
UniProt Protein Details:

Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Oncoprotein; Kinase, protein; Other group; AUR family

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: microtubule cytoskeleton; germinal vesicle; axon hillock; centrosome; pronucleus; spindle microtubule; perinuclear region of cytoplasm; spindle; midbody; spindle midzone; spindle pole centrosome; cytosol; nucleus; condensed nuclear chromosome, pericentric region

Molecular Function:protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: spindle stabilization; mitosis; regulation of centrosome cycle; positive regulation of mitosis; protein amino acid autophosphorylation; regulation of protein stability; regulation of cytokinesis; protein amino acid phosphorylation; mitotic centrosome separation; mitotic spindle organization and biogenesis; centrosome localization; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein binding; spindle assembly involved in female meiosis I; negative regulation of apoptosis; anterior/posterior axis specification

Disease: Colorectal Cancer

NCBI Summary:The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O14965
NCBI GenInfo Identifier:27923855
NCBI Gene ID:6790
NCBI Accession:O14965.2
UniProt Secondary Accession:O14965,O60445, O75873, Q9BQD6, Q9UPG5, E1P5F9,
UniProt Related Accession:O14965
Molecular Weight:403
NCBI Full Name:Aurora kinase A
NCBI Synonym Full Names:aurora kinase A
NCBI Official Symbol:AURKA  
NCBI Official Synonym Symbols:AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47  
NCBI Protein Information:aurora kinase A; ARK-1; hARK1; aurora 2; Aurora-A kinase; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; breast-tumor-amplified kinase; serine/threonine-protein kinase 6; serine/threonine protein kinase 15; serine/threonine-protein kinase 15; serine/threonine-protein kinase aurora-A; protein phosphatase 1, regulatory subunit 47
UniProt Protein Name:Aurora kinase A
UniProt Synonym Protein Names:Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A
Protein Family:Aurora kinase
UniProt Gene Name:AURKA  
UniProt Entry Name:AURKA_HUMAN
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