Human ALKBH3 Recombinant Protein (RPPB1398)

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SKU:
RPPB1398
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
ALKBH3
Synonyms:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
Synonyms:
Alkylated DNA repair protein alkB homolog 3
Synonyms:
DEPC-1
Synonyms:
Prostate cancer antigen 1
Source:
Escherichia Coli
Uniprot:
Q96Q83
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Description

Product Name:Human ALKBH3 Recombinant Protein
Product Code:RPPB1398
Size:20µg
Species:Human
Target:ALKBH3
Synonyms:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, ALKBH3, ABH3, DEPC1, PCA1, FLJ43614, MGC118790, MGC118792, MGC118793.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ALKBH3 solution 1mg/ml contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW

ALKBH3 belongs to the ALKB family of proteins and acts as a dioxygenase with a preference for RNA and single stranded DNA substrates. ALKBH3 protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a broad array of tissues and localizes to the cytoplasm and the nucleus.

ALKBH3 Human Recombinant fused to 39 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 325 amino acids (1-286 a.a.) and having a molecular mass of 37.9kDa. The ALKBH3 is purified by proprietary chromatographic techniques.

UniProt Protein Function:Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
NCBI Summary:The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
UniProt Code:Q96Q83
NCBI GenInfo Identifier:74752087
NCBI Gene ID:221120
NCBI Accession:Q96Q83.1
UniProt Secondary Accession:Q96Q83,Q3SYI0, Q6NX57, Q96BU8, A6NDJ1,
UniProt Related Accession:Q96Q83
Molecular Weight:19,310 Da
NCBI Full Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
NCBI Synonym Full Names:alkB homolog 3, alpha-ketoglutaratedependent dioxygenase
NCBI Official Symbol:ALKBH3  
NCBI Official Synonym Symbols:ABH3; PCA1; DEPC1; hABH3; DEPC-1  
NCBI Protein Information:alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Protein Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Synonym Protein Names:Alkylated DNA repair protein alkB homolog 3
UniProt Gene Name:ALKBH3  
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