The Human Alb (Albumin) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of albumin levels in human serum and plasma samples. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Albumin is a key protein in the body responsible for maintaining oncotic pressure and transporting various molecules, such as hormones and drugs. Changes in albumin levels can indicate various health conditions, including liver or kidney diseases, malnutrition, and inflammatory disorders.
By using the Human Alb CLIA Kit, researchers can effectively measure albumin levels in clinical samples, aiding in the diagnosis and monitoring of various diseases. This kit offers a simple and efficient way to study the role of albumin in health and disease, providing valuable insights for potential therapeutic interventions.
Product Name:
Human ALB (Albumin) CLIA Kit
SKU:
HUES01151
Target:
Human ALB (Albumin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ALB. You can calculate the concentration of Human ALB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
88-102
89-100
Average (%)
108
95
94
1:4
Range (%)
101-114
95-109
90-103
Average (%)
107
103
96
1:8
Range (%)
88-100
88-101
90-103
Average (%)
94
93
97
1:16
Range (%)
86-98
90-107
103-118
Average (%)
91
98
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
100-111
105
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.31
444.15
1789.0
171.78
400.53
1841.83
Standard deviation
16.45
35.22
192.14
20.48
32.88
130.77
C V (%)
8.69
7.93
10.74
11.92
8.21
7.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ALB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ALB in samples. No significant cross-reactivity or interference between Human ALB and analogues was observed.
