Human ADH5 Recombinant Protein (RPPB1356)

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SKU:
RPPB1356
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
ADH5
Synonyms:
Alcohol dehydrogenase 5 (class III) chi polypeptide
Synonyms:
Alcohol dehydrogenase class chi chain
Synonyms:
Glutathione-dependent formaldehyde dehydrogenase
Synonyms:
S-(hydroxymethyl) glutathione dehydrogenase
Source:
Escherichia Coli
Uniprot:
P11766
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Description

Product Name:Human ADH5 Recombinant Protein
Product Code:RPPB1356
Size:20µg
Species:Human
Target:ADH5
Synonyms:Alcohol dehydrogenase 5 (class III) chi polypeptide, Alcohol dehydrogenase class chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl) glutathione dehydrogenase, FDH, ADHX, ADH-3, FALDH, GSH-FDH, GSNOR, EC 1.1.1.1, EC 1.1.1.284.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ADH5 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMANEVI KCKAAVAWEA GKPLSIEEIE VAPPKAHEVR IKIIATAVCH TDAYTLSGAD PEGCFPVILG HEGAGIVESV GEGVTKLKAG DTVIPLYIPQ CGECKFCLNP KTNLCQKIRV TQGKGLMPDG TSRFTCKGKT ILHYMGTSTF SEYTVVADIS VAKIDPLAPL DKVCLLGCGI STGYGAAVNT AKLEPGSVCA VFGLGGVGLA VIMGCKVAGA SRIIGVDINK DKFARAKEFG ATECINPQDF SKPIQEVLIE MTDGGVDYSF ECIGNVKVMR AALEACHKGW GVSVVVGVAA SGEEIATRPF QLVTGRTWKG TAFGGWKSVE SVPKLVSEYM SKKIKVDEFV THNLSFDEIN KAFELMHSGK SIRTVVKI

ADH5 belongs to the alcohol dehydrogenase family which metabolizes a large selection of substrates, such as retinol, ethanol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. ADH5 has practically no activity for ethanol oxidation, but displays high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between glutathione and formaldehyde. ADH5 enzyme is a key factor of cellular metabolism for the removal of formaldehyde, a powerful aggravating and alerting mediator which causes pharyngitis, lacrymation, rhinitis and contact dermatitis.

ADH5 Recombinant produced in E. coli is a single polypeptide chain containing 398 amino acids (1-374) and having a molecular mass of 42.3kDa.ADH5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ADH5: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
UniProt Protein Details:

Protein type:Energy Metabolism - methane; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Lipid Metabolism - fatty acid; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.284

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mitochondrion; nucleus

Molecular Function:protein homodimerization activity; electron carrier activity; zinc ion binding; formaldehyde dehydrogenase activity; alcohol dehydrogenase activity; S-(hydroxymethyl)glutathione dehydrogenase activity; fatty acid binding

Biological Process: response to nitrosative stress; respiratory system process; ethanol catabolic process; formaldehyde catabolic process; peptidyl-cysteine S-nitrosylation; response to redox state; response to lipopolysaccharide; retinoid metabolic process; ethanol oxidation; positive regulation of blood pressure; aging

NCBI Summary:This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
UniProt Code:P11766
NCBI GenInfo Identifier:113408
NCBI Gene ID:128
NCBI Accession:P11766.4
UniProt Related Accession:P11766
Molecular Weight:
NCBI Full Name:Alcohol dehydrogenase class-3
NCBI Synonym Full Names:alcohol dehydrogenase 5 (class III), chi polypeptide
NCBI Official Symbol:ADH5  
NCBI Official Synonym Symbols:FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p  
NCBI Protein Information:alcohol dehydrogenase class-3
UniProt Protein Name:Alcohol dehydrogenase class-3
UniProt Synonym Protein Names:Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-); FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
UniProt Gene Name:ADH5  
UniProt Entry Name:ADHX_HUMAN
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