The Human ACVA (Activin A) ELISA Kit is specifically designed for the precise measurement of Activin A levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it an excellent choice for a variety of research purposes.Activin A is a key protein that plays a vital role in various biological processes, including cell growth, differentiation, and inflammation. Its dysregulation has been linked to numerous diseases, such as cancer, fibrosis, and autoimmune disorders.
As a result, Activin A serves as a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.With the Human ACVA ELISA Kit, researchers can accurately quantify Activin A levels in biological samples, providing invaluable insights into the underlying mechanisms of disease and offering new possibilities for diagnosis and treatment.
Product Name:
Human ACVA (Activin A) ELISA Kit
SKU:
HUES01290
Target:
Human ACVA (Activin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACV-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACV-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACV-A. You can calculate the concentration of Human ACV-A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
97-110
99-116
Average (%)
95
104
106
1:4
Range (%)
85-98
87-98
88-100
Average (%)
92
92
94
1:8
Range (%)
87-99
85-97
85-97
Average (%)
93
90
92
1:16
Range (%)
88-102
88-100
85-99
Average (%)
93
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
254.33
695.45
2237.4
262.87
731.39
2447.57
Standard deviation
15.01
32.62
89.05
13.77
38.98
102.31
C V (%)
5.9
4.69
3.98
5.24
5.33
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ACV-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACV-A in samples. No significant cross-reactivity or interference between Human ACV-A and analogues was observed.
