Human Activating Transcription Factor 3 (ATF3) ELISA Kit
The Human Activating Transcription Factor 3 (ATF3) ELISA Kit is a powerful tool for researchers looking to accurately measure ATF3 levels in human samples. ATF3 is a transcription factor that plays a key role in various cellular processes, including inflammation, apoptosis, and stress responses. By accurately measuring ATF3 levels in serum, plasma, and cell culture supernatants, researchers can gain valuable insights into the role of ATF3 in various disease states and potential therapeutic targets.
This ELISA kit boasts high sensitivity and specificity, ensuring reliable and reproducible results. Its user-friendly design makes it suitable for a wide range of research applications, allowing researchers to study the impact of ATF3 on conditions such as cancer, inflammatory disorders, and neurodegenerative diseases. Overall, the Human ATF3 ELISA Kit is an essential tool for researchers interested in understanding the role of ATF3 in human health and disease.
Product Name:
Human Activating Transcription Factor 3 (ATF3) ELISA Kit
Product Code:
HUDL00271
Size:
96 Assays
Target:
ATF3
Synonyms:
Cyclic AMP-dependent Transcription Factor ATF3
Detection Method:
Sandwich
Range:
15.6-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
