Human ABCA1 (ATP Binding Cassette Transporter A1) ELISA Kit
The Human ABCA1 (ATP Binding Cassette Transporter A1) ELISA Kit is a powerful tool for researchers looking to accurately quantify ABCA1 levels in human samples. This kit is specifically designed for the precise detection of ABCA1 in serum, plasma, and cell culture supernatants, providing high sensitivity and specificity for reliable and reproducible results.ABCA1 is an important transporter protein that plays a key role in cellular cholesterol efflux and high-density lipoprotein (HDL) biogenesis.
Dysregulation of ABCA1 has been associated with various lipid metabolism disorders, making it a valuable target for research on cardiovascular diseases, atherosclerosis, and other related conditions.With its easy-to-use format and proven performance, the Human ABCA1 ELISA Kit is an essential tool for investigating the function and regulation of ABCA1 in health and disease. Upgrade your research with this advanced kit and unlock new insights into the role of ABCA1 in human biology.
Product Name:
Human ABCA1 (ATP Binding Cassette Transporter A1) ELISA Kit
SKU:
HUES01725
Target:
Human ABCA1 (ATP Binding Cassette Transporter A1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ABCA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ABCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ABCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ABCA1. You can calculate the concentration of Human ABCA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
88-100
87-97
Average (%)
96
94
92
1:4
Range (%)
94-110
80-93
84-95
Average (%)
100
86
89
1:8
Range (%)
88-100
80-91
85-95
Average (%)
94
86
90
1:16
Range (%)
94-109
81-93
81-95
Average (%)
99
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.35
3.56
0.51
1.23
3.38
Standard deviation
0.02
0.06
0.11
0.03
0.07
0.14
C V (%)
4.26
4.44
3.09
5.88
5.69
4.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ABCA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ABCA1 in samples. No significant cross-reactivity or interference between Human ABCA1 and analogues was observed.
