HSD3B7 Antibody (PACO53546)
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주문- SKU:
- PACO53546
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | HSD3B7 Antibody (PACO53546) |
Antibody SKU: | PACO53546 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human 3 beta-hydroxysteroid dehydrogenase type 7 protein (131-197AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Mouse liver tissue, Mouse kidney tissue. All lanes: HSD3B7 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 22 kDa. Observed band size: 42 kDa. | |
Immunofluorescent analysis of HepG2 cells using PACO53546 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid, synthesis. Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. |
Synonyms: | 3 beta-hydroxysteroid dehydrogenase type 7 (3 beta-hydroxysteroid dehydrogenase type VII) (3-beta-HSD VII) (3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase) (C(27) 3-beta-HSD) (EC 1.1.1) (Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase) (EC 1.1.1.181), HSD3B7 |
UniProt Protein Function: | HSD3B7: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1); also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis. Belongs to the 3-beta-HSD family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; EC 1.1.1.181; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: endoplasmic reticulum membrane; integral to membrane Molecular Function:3-beta-hydroxy-delta5-steroid dehydrogenase activity; cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity; protein binding Biological Process: bile acid biosynthetic process; bile acid metabolic process Disease: Bile Acid Synthesis Defect, Congenital, 1 |
NCBI Summary: | This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] |
UniProt Code: | Q9H2F3 |
NCBI GenInfo Identifier: | 47605550 |
NCBI Gene ID: | 80270 |
NCBI Accession: | Q9H2F3.2 |
UniProt Secondary Accession: | Q9H2F3,Q96M28, Q9BSN9, |
UniProt Related Accession: | Q9H2F3 |
Molecular Weight: | 21,322 Da |
NCBI Full Name: | 3 beta-hydroxysteroid dehydrogenase type 7 |
NCBI Synonym Full Names: | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 |
NCBI Official Symbol: | HSD3B7 |
NCBI Official Synonym Symbols: | CBAS1; PFIC4; SDR11E3 |
NCBI Protein Information: | 3 beta-hydroxysteroid dehydrogenase type 7 |
UniProt Protein Name: | 3 beta-hydroxysteroid dehydrogenase type 7 |
UniProt Synonym Protein Names: | 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase (EC:1.1.1.-); C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase (EC:1.1.1.181) |
Protein Family: | 3 beta-hydroxysteroid dehydrogenase |
UniProt Gene Name: | HSD3B7 |
UniProt Entry Name: | 3BHS7_HUMAN |
Antibodies | ELISA Kits |
Anti-HSD3B7 Antibody (CAB17777) | Human HSD3B7 (3 beta-hydroxysteroid dehydrogenase type 7) ELISA Kit (HUFI03479) |
HSD3B7 Antibody (PACO06208) | |
HSD3B7 Antibody (PACO09814) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |