HACE1 Antibody (PACO57320)
- SKU:
- PACO57320
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | HACE1 Antibody (PACO57320) |
Antibody SKU: | PACO57320 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human E3 ubiquitin-protein ligase HACE1 protein (333-442AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57320 diluted at 1:600 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO57320 diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. |
Synonyms: | E3 ubiquitin-protein ligase HACE1 (EC 2.3.2.26) (HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1) (HECT-type E3 ubiquitin transferase HACE1), HACE1, KIAA1320 |
UniProt Protein Function: | HAS1: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Also able to catalyze the synthesis of chito- oligosaccharide depending on the substrate. Belongs to the NodC/HAS family. |
UniProt Protein Details: | Protein type:Cell adhesion; EC 2.4.1.212; Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass; Transferase Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: cytoplasm; integral to plasma membrane; plasma membrane Molecular Function:hyaluronan synthase activity Biological Process: carbohydrate metabolic process; cell adhesion; extracellular polysaccharide biosynthetic process; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; hyaluronan biosynthetic process; hyaluronan metabolic process |
NCBI Summary: | This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016] |
UniProt Code: | Q8IYU2 |
NCBI GenInfo Identifier: | 116875852 |
NCBI Gene ID: | 57531 |
NCBI Accession: | NP_065822 |
UniProt Related Accession: | Q8IYU2 |
Molecular Weight: | 76kDa |
NCBI Full Name: | E3 ubiquitin-protein ligase HACE1 isoform a |
NCBI Synonym Full Names: | HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 |
NCBI Official Symbol: | HACE1 |
NCBI Official Synonym Symbols: | SPPRS |
NCBI Protein Information: | E3 ubiquitin-protein ligase HACE1 |
UniProt Protein Name: | Hyaluronan synthase 1 |
UniProt Synonym Protein Names: | Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HuHAS1 |
Protein Family: | Hyaluronan synthase |
UniProt Gene Name: | HAS1 |
UniProt Entry Name: | HYAS1_HUMAN |
Antibodies | ELISA Kits |
Anti-HACE1 Antibody (CAB9593) | Human HACE1 ELISA Kit |
Human E3 ubiquitin-protein ligase HACE1 (HACE1) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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