GLI2 Antibody (PACO62911)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO62911
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GLI2 Antibody (PACO62911) |
Antibody SKU: | PACO62911 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence from Human Zinc finger protein GLI2 protein (2-20AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO62911 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate. All lanes: GLI2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 168, 134, 132, 89, 87 kDa. Observed band size: 168 kDa. |
Background: | Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development. |
Synonyms: | Zinc finger protein GLI2 (GLI family zinc finger protein 2) (Tax helper protein), GLI2, THP |
UniProt Protein Function: | Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor. Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856). |
NCBI Summary: | This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008] |
UniProt Code: | P10070 |
NCBI GenInfo Identifier: | 215274258 |
NCBI Gene ID: | 2736 |
NCBI Accession: | P10070.4 |
UniProt Related Accession: | P10070 |
Molecular Weight: | 168kDa |
NCBI Full Name: | Zinc finger protein GLI2 |
NCBI Synonym Full Names: | GLI family zinc finger 2 |
NCBI Official Symbol: | GLI2 |
NCBI Official Synonym Symbols: | CJS; HPE9; PHS2; THP1; THP2 |
NCBI Protein Information: | zinc finger protein GLI2 |
UniProt Protein Name: | Zinc finger protein GLI2 |
UniProt Synonym Protein Names: | GLI family zinc finger protein 2; Tax helper protein |
Protein Family: | Zinc finger protein |
UniProt Gene Name: | GLI2 |
UniProt Entry Name: | GLI2_HUMAN |
Antibodies |
Anti-GLI2 Antibody (CAB16863) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |