General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
The 8-epi-Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit from Assay Genie is a cutting-edge tool for the precise measurement of 8-epi-PGF2a levels in various samples such as serum, plasma, and tissue culture supernatants. With unparalleled sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable asset for researchers in a variety of fields.8-epi-PGF2a is a key biomarker for oxidative stress and inflammation, playing a crucial role in numerous pathological conditions, including cardiovascular diseases, respiratory disorders, and neurodegenerative diseases.
By accurately detecting and quantifying 8-epi-PGF2a levels, researchers can gain valuable insights into disease mechanisms, paving the way for the development of targeted therapeutic strategies.Trust Assay Genie's 8-epi-Prostaglandin F2 Alpha ELISA Kit to enhance your research and accelerate progress towards a better understanding of oxidative stress-related disorders.
Product Name:
General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit
Product Code:
UNDL00004
Size:
96 Assays
Synonyms:
8-EpiPGF2-?, 8epi-PGF2a
Detection Method:
Competition
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
