EMP2 Antibody (PACO58737)
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주문- SKU:
- PACO58737
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | EMP2 Antibody (PACO58737) |
Antibody SKU: | PACO58737 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Epithelial membrane protein 2 protein (22-66AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO58737 diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO58737 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation. Facilitates surface trafficking and formation of lipid rafts bearing GPI-anchor proteins (By similarity). Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (By similarity). Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion. Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation. Regulates cell migration and cell contraction through PTK2 and SRC activation. Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2. Positively regulates cell proliferation. Plays a role during cell death and cell blebbing. Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway. Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3. May play a role in glomerular filtration (By similarity). |
Synonyms: | Epithelial membrane protein 2 (EMP-2) (Protein XMP), EMP2, XMP |
UniProt Protein Function: | EMP2: Belongs to the PMP-22/EMP/MP20 family. |
UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 16p13.2 Cellular Component: apical part of cell; caveola; cell surface; cytoplasm; cytoplasmic vesicle; Golgi apparatus; lipid raft; nucleus; plasma membrane Molecular Function:integrin binding; kinase binding; protein binding; protein kinase binding Biological Process: actin filament organization; activation of protein kinase activity; bleb formation; blood vessel endothelial cell migration; cell adhesion; cell death; cell migration; cell proliferation; cell-matrix adhesion; early endosome to late endosome transport; embryo implantation; lipid raft formation; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; regulation of angiogenesis; regulation of glomerular filtration; regulation of kinase activity; T cell mediated cytotoxicity Disease: Nephrotic Syndrome, Type 10 |
NCBI Summary: | This gene encodes a tetraspan protein of the PMP22/EMP family. The encoded protein regulates cell membrane composition. It has been associated with various functions including endocytosis, cell signaling, cell proliferation, cell migration, cell adhesion, cell death, cholesterol homeostasis, urinary albumin excretion, and embryo implantation. It is known to negatively regulate caveolin-1, a scaffolding protein which is the main component of the caveolae plasma membrane invaginations found in most cell types. Through activation of PTK2 it positively regulates vascular endothelial growth factor A. It also modulates the function of specific integrin isomers in the plasma membrane. Up-regulation of this gene has been linked to cancer progression in multiple different tissues. Mutations in this gene have been associated with nephrotic syndrome type 10 (NPHS10). [provided by RefSeq, Mar 2015] |
UniProt Code: | P54851 |
NCBI GenInfo Identifier: | 1706643 |
NCBI Gene ID: | 2013 |
NCBI Accession: | P54851.1 |
UniProt Secondary Accession: | P54851,B2R7V6, D3DUF8, |
UniProt Related Accession: | P54851 |
Molecular Weight: | 19,199 Da |
NCBI Full Name: | Epithelial membrane protein 2 |
NCBI Synonym Full Names: | epithelial membrane protein 2 |
NCBI Official Symbol: | EMP2 |
NCBI Official Synonym Symbols: | XMP |
NCBI Protein Information: | epithelial membrane protein 2 |
UniProt Protein Name: | Epithelial membrane protein 2 |
UniProt Synonym Protein Names: | Protein XMP |
Protein Family: | Epithelial membrane protein |
UniProt Gene Name: | EMP2 |
UniProt Entry Name: | EMP2_HUMAN |
Antibodies |
Anti-EMP2 Antibody (CAB16327) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |