E.Coli GlpK E. coli Recombinant Protein (RPPB2544)

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SKU:
RPPB2544
Product type:
Recombinant Protein
Size:
20ug
Species:
E.Coli
Target:
GlpK E. coli
Synonyms:
Glycerolkinase
Synonyms:
glycerol 3-phosphotransferase
Synonyms:
Glycerokinase
Synonyms:
GK
Source:
Escherichia Coli
Uniprot:
P0A6F3
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Description

Product Name:E.Coli GlpK E. coli Recombinant Protein
Product Code:RPPB2544
Size:20µg
Species:E.Coli
Target:GlpK E. coli
Synonyms:Glycerolkinase, glycerol 3-phosphotransferase, Glycerokinase, GK.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:GlpK protein solution (1mg/ml) containing Phosphatebuffered saline (pH7.4),10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHHSSGLVPRGSH MGSMTEKKYIVALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAAIGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILDHVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPEVRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTGEVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFGLTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVREVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE

GlpKalso known as glycerol kinase, is a member of the FGGY kinase family. GlpKcatalyzes the transfer of a phosphate group from ATP to glycerol, therebyforming glycerol phosphate. Furthermore, this intermediate can then beconverted to dihydroxyacetone phosphate (DHAP), which is utilized in eitherglycolysis or gluconeogenesis. The activity of GlpK is affected by numerous metabolites.The non-competitive allosteric inhibition by fructose 1,6-bisphosphate (FBP) triggersmodifications in the quaternary structure of Glpk.

GlpK E. Coli Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 525 amino acids (1-502 a.a) and having a molecular mass of 58.6 kDa.GlpK is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP.
UniProt Protein Details:
NCBI Summary:The enzyme can undergo a reversible subunit dissociation between tetramer and dimer . [More information is available at EcoCyc: EG10398].
UniProt Code:P0A6F3
NCBI GenInfo Identifier:16131764
NCBI Gene ID:948423
NCBI Accession:NP_418361.1
UniProt Secondary Accession:P0A6F3,P08859, Q2M8M2, Q59381
UniProt Related Accession:P0A6F3
Molecular Weight:56,231 Da
NCBI Full Name:glycerol kinase
NCBI Synonym Full Names:
NCBI Official Symbol:glpK  
NCBI Official Synonym Symbols:ECK3918; JW3897  
NCBI Protein Information:glycerol kinase
UniProt Protein Name:Glycerol kinase
UniProt Synonym Protein Names:ATP:glycerol 3-phosphotransferase
Protein Family:Glycerol kinase
UniProt Gene Name:glpK  
UniProt Entry Name:GLPK_ECOLI
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