DsbA Recombinant Protein (RPPB1585)

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SKU:
RPPB1585
Product type:
Recombinant Protein
Size:
50ug
Target:
DsbA
Synonyms:
DsbA
Synonyms:
Thiol:disulfide interchange protein dsbA
Source:
Escherichia Coli
Uniprot:
P0AEG4
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Description

Product Name:DsbA Recombinant Protein
Product Code:RPPB1585
Size:50µg
Target:DsbA
Synonyms:DsbA, Thiol:disulfide interchange protein dsbA.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride.
Solubility:It is recommended to reconstitute the lyophilized DsbA in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DsbA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:The sequence of the first five N-terminal amino acidswas determined and was found to be Met-Ly-Lys-Ala-Trp

DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.

Disulfide Oxidoreductase produced in E.Coli is a periplasmic protein isolated from E. coli, containing 208 amino acids having a molecular mass of 23,149 Dalton. The DsbA is purified by proprietary chromatographic techniques.

UniProt Protein Function:Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
UniProt Protein Details:
NCBI Summary:DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].
UniProt Code:P0AEG4
NCBI GenInfo Identifier:16131701
NCBI Gene ID:948353
NCBI Accession:NP_418297.1
UniProt Secondary Accession:P0AEG4,P24991, Q2M8F8, Q46951, Q46952
UniProt Related Accession:P0AEG4
Molecular Weight:23,105 Da
NCBI Full Name:periplasmic protein disulfide isomerase I
NCBI Synonym Full Names:
NCBI Official Symbol:dsbA  
NCBI Official Synonym Symbols:dsf; ECK3852; iarA; JW3832; ppfA  
NCBI Protein Information:periplasmic protein disulfide isomerase I
UniProt Protein Name:Thiol:disulfide interchange protein DsbA
UniProt Synonym Protein Names:
Protein Family:Double-stranded DNA-binding protein
UniProt Gene Name:dsbA  
UniProt Entry Name:DSBA_ECOLI
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