DNAJA1 Antibody (PACO45770)
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주문- SKU:
- PACO45770
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | DNAJA1 Antibody (PACO45770) |
Antibody SKU: | PACO45770 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human DnaJ homolog subfamily A member 1 protein (8-106AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: DNAJA1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45, 38 kDa. Observed band size: 45, 38 kDa.. |
![]() | Immunofluorescence staining of HepG2 cells with PACO45770 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO45770 diluted at 1:1000 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV. |
Synonyms: | DnaJ homolog subfamily A member 1 (DnaJ protein homolog 2) (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2), DNAJA1, DNAJ2 HDJ2 HSJ2 HSPF4 |
UniProt Protein Function: | DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria. |
UniProt Protein Details: | Protein type:Chaperone; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; cytosol; nucleus; ubiquitin ligase complex Molecular Function:protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; chaperone binding; metal ion binding; ubiquitin protein ligase binding; unfolded protein binding; Hsp70 protein binding; ATP binding Biological Process: negative regulation of JNK activity; protein folding; positive regulation of apoptosis; DNA damage response, detection of DNA damage; response to unfolded protein; regulation of protein transport; sperm motility; response to heat; androgen receptor signaling pathway; spermatogenesis; protein refolding; negative regulation of protein ubiquitination; negative regulation of apoptosis |
UniProt Code: | P31689 |
NCBI GenInfo Identifier: | 1706474 |
NCBI Gene ID: | 3301 |
NCBI Accession: | P31689.2 |
UniProt Secondary Accession: | P31689,Q5T7Q0, Q86TL9, |
UniProt Related Accession: | P31689 |
Molecular Weight: | 397 |
NCBI Full Name: | DnaJ homolog subfamily A member 1 |
NCBI Synonym Full Names: | DnaJ (Hsp40) homolog, subfamily A, member 1 |
NCBI Official Symbol: | DNAJA1 |
NCBI Official Synonym Symbols: | DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2 |
NCBI Protein Information: | dnaJ homolog subfamily A member 1; HSJ-2; human DnaJ protein 2; heat shock protein J2; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein, DNAJ-like 2; neural precursor cell expressed, developmentally down-regulated 7 |
UniProt Protein Name: | DnaJ homolog subfamily A member 1 |
UniProt Synonym Protein Names: | DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2 |
UniProt Gene Name: | DNAJA1 |
UniProt Entry Name: | DNJA1_HUMAN |
Antibodies |
Anti-DNAJA1 Antibody (CAB11602) |
Anti-DNAJA1 Antibody (CAB19865)[KO Validated] |
DNAJA1 Antibody (PACO01876) |
DNAJA1 Antibody (PACO08870) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |