DFFA Antibody (PACO43939)
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주문- SKU:
- PACO43939
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | DFFA Antibody (PACO43939) |
Antibody SKU: | PACO43939 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human DNA fragmentation factor subunit α protein (1-331AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: DFFA antibody at 10µg/ml. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: PC-3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 37, 30 kDa. Observed band size: 37 kDa. |
![]() | Immunohistochemistry of paraffin-embedded human colon cancer using PACO43939 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human breast cancer using PACO43939 at dilution of 1:100. |
Background: | Inhibitor of the caspase-activated DNase (DFF40). |
Synonyms: | DNA fragmentation factor subunit α (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) (ICAD), DFFA, DFF1 DFF45 |
UniProt Protein Function: | DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Hydrolase Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cytosol; lipid particle; nuclear chromatin; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding; protein domain specific binding Biological Process: DNA fragmentation during apoptosis; negative regulation of deoxyribonuclease activity |
NCBI Summary: | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | O00273 |
NCBI GenInfo Identifier: | 2810997 |
NCBI Gene ID: | 1676 |
NCBI Accession: | O00273.1 |
UniProt Secondary Accession: | O00273,Q5T6G5, Q5T6G6, Q96I97, Q9Y6C6, |
UniProt Related Accession: | O00273 |
Molecular Weight: | 37kDa |
NCBI Full Name: | DNA fragmentation factor subunit alpha |
NCBI Synonym Full Names: | DNA fragmentation factor subunit alpha |
NCBI Official Symbol: | DFFA |
NCBI Official Synonym Symbols: | DFF1; ICAD; DFF-45 |
NCBI Protein Information: | DNA fragmentation factor subunit alpha |
UniProt Protein Name: | DNA fragmentation factor subunit alpha |
UniProt Synonym Protein Names: | DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD |
Protein Family: | DNA fragmentation factor |
UniProt Gene Name: | DFFA |
Antibodies |
Anti-DFFA Antibody (CAB1032)[KO Validated] |
Anti-DFFA Antibody (CAB12431) |
DFFA Antibody (PACO00029) |
DFFA Antibody (PACO06573) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |