DAZAP2 Antibody (PACO60028)
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주문- SKU:
- PACO60028
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | DAZAP2 Antibody (PACO60028) |
Antibody SKU: | PACO60028 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human DAZ-associated protein 2 protein (1-168AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: DAZAP2 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 18, 17, 16, 14, 10, 23 kDa. Observed band size: 18 kDa. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO60028 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO60028 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | nuclear speck, identical protein binding, WW domain binding |
Synonyms: | DAZ-associated protein 2 (Deleted in azoospermia-associated protein 2), DAZAP2, KIAA0058 |
UniProt Protein Function: | DAZAP2: 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 12q12 Molecular Function:protein binding; WW domain binding |
NCBI Summary: | This gene encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability in splicing factor containing nuclear speckles. The encoded protein may function in various biological and pathological processes including spermatogenesis, cell signaling and transcription regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
UniProt Code: | Q15038 |
NCBI GenInfo Identifier: | 44887865 |
NCBI Gene ID: | 9802 |
NCBI Accession: | Q15038.1 |
UniProt Secondary Accession: | Q15038,A8K254, B4DDT5, B4E1G3, C9JA96, C9JP84, E9PB45 F8VU62, |
UniProt Related Accession: | Q15038 |
Molecular Weight: | 22,823 Da |
NCBI Full Name: | DAZ-associated protein 2 |
NCBI Synonym Full Names: | DAZ associated protein 2 |
NCBI Official Symbol: | DAZAP2 |
NCBI Official Synonym Symbols: | PRTB |
NCBI Protein Information: | DAZ-associated protein 2 |
UniProt Protein Name: | DAZ-associated protein 2 |
UniProt Synonym Protein Names: | Deleted in azoospermia-associated protein 2 |
Protein Family: | DAZ-associated protein |
UniProt Gene Name: | DAZAP2 |
UniProt Entry Name: | DAZP2_HUMAN |
Antibodies |
Anti-DAZAP2 Antibody (CAB14266) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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