DAD1 Antibody (PACO31624)
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주문- SKU:
- PACO31624
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | DAD1 Antibody (PACO31624) |
Antibody SKU: | PACO31624 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein (1-113AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: DAD1 antibody at 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 13 kDa . | |
Immunofluorescent analysis of HepG2 cells using PACO31624 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human prostate tissue using PACO31624 at dilution of 1:100. |
Background: | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis. |
Synonyms: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (Oligosaccharyl transferase subunit DAD1) (Defender against cell death 1) (DAD-1), DAD1 |
UniProt Protein Function: | DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family. |
UniProt Protein Details: | Protein type:Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.99.18; Transferase; Membrane protein, multi-pass; Membrane protein, integral; Apoptosis Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complex; integral to membrane Molecular Function:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity Biological Process: response to drug; blastocyst development; cellular protein metabolic process; apoptosis; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to nutrient; negative regulation of apoptosis |
NCBI Summary: | DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008] |
UniProt Code: | P61803 |
NCBI GenInfo Identifier: | 48428858 |
NCBI Gene ID: | 1603 |
NCBI Accession: | P61803.3 |
UniProt Secondary Accession: | P61803,O08552, O70364, P46966, P46968, Q6FGA3, Q96GB7 D3DS25, |
UniProt Related Accession: | P61803 |
Molecular Weight: | 12,497 Da |
NCBI Full Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 |
NCBI Synonym Full Names: | defender against cell death 1 |
NCBI Official Symbol: | DAD1 |
NCBI Official Synonym Symbols: | OST2 |
NCBI Protein Information: | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic) |
UniProt Protein Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 |
UniProt Synonym Protein Names: | Defender against cell death 1; DAD-1 |
Protein Family: | DASH complex |
UniProt Gene Name: | DAD1 |
UniProt Entry Name: | DAD1_HUMAN |