COPG2 Antibody (PACO58480)
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주문- SKU:
- PACO58480
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | COPG2 Antibody (PACO58480) |
Antibody SKU: | PACO58480 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Coatomer subunit gamma-2 protein (676-823AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO58480 diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of MCF-7 cells with PACO58480 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO58480 diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). |
Synonyms: | Coatomer subunit gamma-2 (Gamma-2-coat protein) (Gamma-2-COP), COPG2 |
UniProt Protein Function: | COPG2: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the COPG family. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Vesicle Chromosomal Location of Human Ortholog: 7q32 Cellular Component: COPI vesicle coat; cytosol Molecular Function:structural molecule activity Biological Process: intra-Golgi vesicle-mediated transport; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER |
UniProt Code: | Q9UBF2 |
NCBI GenInfo Identifier: | 13124090 |
NCBI Gene ID: | 26958 |
NCBI Accession: | Q9UBF2.1 |
UniProt Related Accession: | Q9UBF2 |
Molecular Weight: | Calculated: 81kDa; 97kDaObserved: 105kDa |
NCBI Full Name: | Coatomer subunit gamma-2 |
NCBI Synonym Full Names: | coatomer protein complex subunit gamma 2 |
NCBI Official Symbol: | COPG2 |
NCBI Official Synonym Symbols: | 2-COP; gamma-2-COP |
NCBI Protein Information: | coatomer subunit gamma-2 |
UniProt Protein Name: | Coatomer subunit gamma-2 |
UniProt Synonym Protein Names: | Gamma-2-coat protein; Gamma-2-COP |
Protein Family: | Coatomer |
UniProt Gene Name: | COPG2 |
UniProt Entry Name: | COPG2_HUMAN |