CCKAR Antibody (PACO55418)
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주문- SKU:
- PACO55418
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | CCKAR Antibody (PACO55418) |
Antibody SKU: | PACO55418 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Cholecystokinin receptor type A protein (235-313AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: A549 whole cell lysate. All lanes: CCKAR antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa. |
![]() | Immunofluorescence staining of Hela cells with PACO55418 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
![]() | IHC image of PACO55418 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. |
Synonyms: | Cholecystokinin receptor type A (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R), CCKAR, CCKRA |
UniProt Protein Function: | CCKAR: Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. |
UniProt Protein Details: | Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: integral to plasma membrane; plasma membrane Biological Process: digestion; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of hormone secretion |
NCBI Summary: | This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine. [provided by RefSeq, Jul 2008] |
UniProt Code: | P32238 |
NCBI GenInfo Identifier: | 416772 |
NCBI Gene ID: | 886 |
NCBI Accession: | P32238.1 |
UniProt Secondary Accession: | P32238,B2R9Z5, |
UniProt Related Accession: | P32238 |
Molecular Weight: | 47,841 Da |
NCBI Full Name: | Cholecystokinin receptor type A |
NCBI Synonym Full Names: | cholecystokinin A receptor |
NCBI Official Symbol: | CCKAR |
NCBI Official Synonym Symbols: | CCK-A; CCK1R; CCKRA; CCK1-R |
NCBI Protein Information: | cholecystokinin receptor type A |
UniProt Protein Name: | Cholecystokinin receptor type A |
UniProt Synonym Protein Names: | Cholecystokinin-1 receptor; CCK1-R |
Protein Family: | Cholecystokinin receptor |
UniProt Gene Name: | CCKAR |
UniProt Entry Name: | CCKAR_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |