The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
Product Name:
Carbonic Anhydrase 4/CA4 Recombinant Protein (RPES2882)
Product Code:
RPES2882
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Carbonic Anhydrase 4, Carbonate Dehydratase IV, Carbonic Anhydrase IV, CA-IV, CA4,CAIV,Car4,RP17
Accession:
NP_000708.1
Sequence:
Met 1-Lys283
Fusion tag:
C-His
Activity:
Measured by its esterase activity. The specific activity is >2 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
31.7 kDa
AP Mol Mass:
30 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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