CACNA1B Antibody (PACO63267)
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주문- SKU:
- PACO63267
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | CACNA1B Antibody (PACO63267) |
Antibody SKU: | PACO63267 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Voltage-dependent N-type calcium channel subunit alpha-1B protein (2132-2339AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO63267 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of SH-SY5Y cells with PACO63267 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons. |
Synonyms: | Voltage-dependent N-type calcium channel subunit alpha-1B (Brain calcium channel III) (BIII) (Calcium channel, L type, alpha-1 polypeptide isoform 5) (Voltage-gated calcium channel subunit α Cav2.2), CACNA1B, CACH5 CACNL1A5 |
NCBI Summary: | The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
UniProt Code: | Q00975 |
NCBI GenInfo Identifier: | 4502523 |
NCBI Gene ID: | 774 |
NCBI Accession: | NP_000709 |
UniProt Related Accession: | Q00975 |
Molecular Weight: | Predicted Molecular Mass: 25.1kDaAccurate Molecular Mass: 28kDa |
NCBI Full Name: | voltage-dependent N-type calcium channel subunit alpha-1B isoform 1 |
NCBI Synonym Full Names: | calcium voltage-gated channel subunit alpha1 B |
NCBI Official Symbol: | CACNA1B |
NCBI Official Synonym Symbols: | BIII; CACNN; DYT23; Cav2.2; NEDNEH; CACNL1A5 |
NCBI Protein Information: | voltage-dependent N-type calcium channel subunit alpha-1B |
UniProt Protein Name: | Voltage-dependent N-type calcium channel subunit alpha-1B |
UniProt Synonym Protein Names: | Brain calcium channel III; BIII; Calcium channel, L type, alpha-1 polypeptide isoform 5; Voltage-gated calcium channel subunit alpha Cav2.2 |
Protein Family: | GTP-binding protein |
UniProt Gene Name: | CACNA1B |
UniProt Entry Name: | CAC1B_HUMAN |