APAF1 Antibody (PACO23092)
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주문- SKU:
- PACO23092
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | APAF1 Antibody (PACO23092) |
Antibody SKU: | PACO23092 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200 |
Species Reactivity: | Human |
Immunogen: | Synthesized peptide derived from human APAF-1-ALT. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from COLO205, using APAF-1-ALT antibody. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF-1-ALT antibody. |
Background: | This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. |
Synonyms: | APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19 |
UniProt Protein Function: | APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Apoptosis Chromosomal Location of Human Ortholog: 12q23.1 Cellular Component: apoptosome; cytosol Molecular Function:nucleotide binding; protein binding Biological Process: apoptosis; caspase activation; caspase activation via cytochrome c; nervous system development; positive regulation of apoptosis; regulation of apoptosis |
NCBI Summary: | This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
UniProt Code: | O14727 |
NCBI GenInfo Identifier: | 20141188 |
NCBI Gene ID: | 317 |
NCBI Accession: | O14727.2 |
UniProt Secondary Accession: | O14727,O43297, Q7Z438, Q9BXZ6, Q9UBZ5, Q9UGN8, Q9UGN9 Q9UGP0, Q9UJ58, Q9UJ59, Q9UJ60, B2RMX8, |
UniProt Related Accession: | O14727 |
Molecular Weight: | 37,976 Da |
NCBI Full Name: | Apoptotic protease-activating factor 1 |
NCBI Synonym Full Names: | apoptotic peptidase activating factor 1 |
NCBI Official Symbol: | APAF1 |
NCBI Official Synonym Symbols: | CED4; APAF-1 |
NCBI Protein Information: | apoptotic protease-activating factor 1 |
UniProt Protein Name: | Apoptotic protease-activating factor 1 |
Protein Family: | Apoptotic protease-activating factor |
UniProt Gene Name: | APAF1 |
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