AP2A2 Antibody (PACO44187)
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주문- SKU:
- PACO44187
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | AP2A2 Antibody (PACO44187) |
Antibody SKU: | PACO44187 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human AP-2 complex subunit alpha-2 protein (590-939AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: AP2A2 antibody at 0.61µg/ml + Mouse brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 104, 105, 74 kDa. Observed band size: 104 kDa.. |
![]() | Immunohistochemistry of paraffin-embedded human testis tissue using PACO44187 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human heart tissue using PACO44187 at dilution of 1:100. |
Background: | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 α subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 α subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 α and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). |
Synonyms: | AP-2 complex subunit alpha-2 (100 kDa coated vesicle protein C) (Adaptor protein complex AP-2 subunit alpha-2) (Adaptor-related protein complex 2 subunit alpha-2) (Alpha-adaptin C) (Alpha2-adaptin) (Clathrin assembly protein complex 2 alpha-C large chain) (Huntingtin yeast partner J) (Huntingtin-interacting protein 9) (HIP-9) (Huntingtin-interacting protein J) (Plasma membrane adaptor HA2/AP2 adaptin α C subunit), AP2A2, ADTAB CLAPA2 HIP9 HYPJ KIAA0899 |
UniProt Protein Function: | AP2A2: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes large subunit family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Adaptor/scaffold; Vesicle Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytoplasmic membrane-bound vesicle; plasma membrane; AP-2 adaptor complex; cytosol Molecular Function:protein binding; lipid binding; protein kinase binding; protein transporter activity Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; axon guidance; intracellular protein transport; synaptic transmission; nerve growth factor receptor signaling pathway; viral reproduction; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus |
UniProt Code: | O94973 |
NCBI GenInfo Identifier: | 12643300 |
NCBI Gene ID: | 161 |
NCBI Accession: | O94973.2 |
UniProt Secondary Accession: | O94973,O75403, Q53ET1, Q96SI8, |
UniProt Related Accession: | O94973 |
Molecular Weight: | |
NCBI Full Name: | AP-2 complex subunit alpha-2 |
NCBI Synonym Full Names: | adaptor-related protein complex 2, alpha 2 subunit |
NCBI Official Symbol: | AP2A2 |
NCBI Official Synonym Symbols: | HIP9; HYPJ; ADTAB; HIP-9; CLAPA2 |
NCBI Protein Information: | AP-2 complex subunit alpha-2; 100 kDa coated vesicle protein C; adapter-related protein complex 2 subunit alpha-2; adaptin, alpha B; adaptor-related protein complex 2 subunit alpha-2; alpha-adaptin C; Huntingtin interacting protein J; alpha2-adaptin; clathrin assembly protein complex 2 alpha-C large chain; clathrin-associated/assembly/adaptor protein, large, alpha 2; huntingtin yeast partner J; huntingtin-interacting protein 9; plasma membrane adaptor HA2/AP2 adaptin alpha C subunit |
UniProt Protein Name: | AP-2 complex subunit alpha-2 |
UniProt Synonym Protein Names: | 100 kDa coated vesicle protein C; Adaptor protein complex AP-2 subunit alpha-2; Adaptor-related protein complex 2 subunit alpha-2; Alpha-adaptin C; Alpha2-adaptin; Clathrin assembly protein complex 2 alpha-C large chain; Huntingtin yeast partner J; Huntingtin-interacting protein 9; HIP-9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit |
Protein Family: | AP-2 complex |
UniProt Gene Name: | AP2A2 |
UniProt Entry Name: | AP2A2_HUMAN |