Anti-YWHAZ Antibody (CAB13370)
- SKU:
- CAB13370
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-YWHAZ Antibody |
| Antibody SKU: | CAB13370 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human YWHAZ (NP_001129171.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HeLa, A-549, MCF7, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human YWHAZ (NP_001129171.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLEK FLIP NASQ AESK VFYL KMKG DYYR YLAE VAAG DDKK GIVD QSQQ AYQE AFEI SKKE MQPT HPIR LGLA LNFS VFYY EILN SPEK ACSL AKTA FDEA IAEL DTLS EESY KDST LIMQ LLRD NLTL WTSD TQGD EAEA GEGG EN |
| Gene ID: | 7534 |
| Uniprot: | P63104 |
| Cellular Location: | Cytoplasm, Melanosome |
| Calculated MW: | 19kDa/27kDa |
| Observed MW: | 28kDa |
| Synonyms: | YWHAZ, 14-3-3-zeta, HEL-S-3, HEL-S-93, HEL4, KCIP-1, YWHAD |
| Background: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| UniProt Protein Function: | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| NCBI Summary: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P63104 |
| NCBI GenInfo Identifier: | 52000887 |
| NCBI Gene ID: | 7534 |
| NCBI Accession: | P63104.1 |
| UniProt Secondary Accession: | P63104,P29213, P29312, Q32P43, Q5XJ08, Q6GPI2, Q6IN74 Q6NUR9, Q6P3U9, Q86V33, A8K1N0, B7Z465, |
| UniProt Related Accession: | P63104 |
| Molecular Weight: | 19,333 Da |
| NCBI Full Name: | 14-3-3 protein zeta/delta |
| NCBI Synonym Full Names: | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta |
| NCBI Official Symbol: | YWHAZ |
| NCBI Official Synonym Symbols: | HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta |
| NCBI Protein Information: | 14-3-3 protein zeta/delta |
| UniProt Protein Name: | 14-3-3 protein zeta/delta |
| UniProt Synonym Protein Names: | Protein kinase C inhibitor protein 1; KCIP-1 |
| Protein Family: | 14-3-3 protein |
| UniProt Gene Name: | YWHAZ |
 | Western blot analysis of extracts of various cell lines, using YWHAZ Antibody (CAB13370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using YWHAZ antibody (CAB13370) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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