Anti-YME1L1 Antibody (CAB10402)
- SKU:
- CAB10402
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-YME1L1 Antibody |
| Antibody SKU: | CAB10402 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human YME1L1 (NP_055078.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human YME1L1 (NP_055078.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFSL SSTV QPQV TVPL SHLI NAFH TPKN TSVS LSGV SVSQ NQHR DVVP EHEA PSSE PSLN LRDL GLSE LKIG QIDQ LVEN LLPG FCKG KNIS SHWH TSHV SAQS FFEN KYGN LDIF STLR SSCL YRHH SRAL QSIC SDLQ YWPV FIQS RGFK TLKS RTRR LQST SERL AETQ NIAP SFVK GFLL RDRG SDVE SLDK LMKT KNIP EAHQ DAFK TGFA EGFL KAQA LTQK TNDS LRRT RLIL |
| Gene ID: | 10730 |
| Uniprot: | Q96TA2 |
| Cellular Location: | Mitochondrion inner membrane |
| Calculated MW: | 75kDa/79kDa/86kDa |
| Observed MW: | 86kDa |
| Synonyms: | YME1L1, FTSH, MEG4, OPA11, PAMP, YME1L |
| Background: | The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | YME1L1: Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Protease; EC 3.4.24.- Chromosomal Location of Human Ortholog: 10p14 Cellular Component: integral to membrane; membrane; mitochondrial inner membrane; mitochondrion; nucleoplasm Molecular Function:ATP binding; ATP-dependent peptidase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity Biological Process: cell proliferation; misfolded or incompletely synthesized protein catabolic process; mitochondrion organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q96TA2 |
| NCBI GenInfo Identifier: | 46397258 |
| NCBI Gene ID: | 10730 |
| NCBI Accession: | Q96TA2.2 |
| UniProt Secondary Accession: | Q96TA2,Q5T8D9, Q9H1Q0, Q9UMR9, B4DNM1, D3DRV8, D3DRV9 |
| UniProt Related Accession: | Q96TA2 |
| Molecular Weight: | 75,982 Da |
| NCBI Full Name: | ATP-dependent zinc metalloprotease YME1L1 |
| NCBI Synonym Full Names: | YME1 like 1 ATPase |
| NCBI Official Symbol: | YME1L1 |
| NCBI Official Synonym Symbols: | FTSH; MEG4; PAMP; YME1L |
| NCBI Protein Information: | ATP-dependent zinc metalloprotease YME1L1 |
| UniProt Protein Name: | ATP-dependent zinc metalloprotease YME1L1 |
| UniProt Synonym Protein Names: | ATP-dependent metalloprotease FtsH1; Meg-4; Presenilin-associated metalloprotease; PAMP; YME1-like protein 1 |
| UniProt Gene Name: | YME1L1 |
| UniProt Entry Name: | YMEL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using YME1L1 antibody (CAB10402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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