Anti-WDR82 항체 (CAB15518)

Antibody Name:	Anti-WDR82 Antibody
Antibody SKU:	CAB15518
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 174-313 of human WDR82 (NP_079498.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse thymus, Mouse brain, Mouse heart, Mouse kidney, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 174-313 of human WDR82 (NP_079498.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YDLR SFDK GPFA TFKM QYDR TCEW TGLK FSND GKLI LIST NGSF IRLI DAFK GVVM HTFG GYAN SKAV TLEA SFTP DSQF IMIG SEDG KIHV WNGE SGIK VAVL DGKH TGPI TCLQ FNPK FMTF ASAC SNMA FWLP TIDD
Gene ID:	80335
Uniprot:	Q6UXN9
Cellular Location:	Nucleus
Calculated MW:	35kDa
Observed MW:	35kDa

Synonyms:	WDR82, MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WDR82A
Background:

UniProt Protein Function:	WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS.
UniProt Protein Details:	Protein type:Methyltransferase Chromosomal Location of Human Ortholog: 3p21.2 Cellular Component: chromatin; histone methyltransferase complex; nuclear chromosome, telomeric region Molecular Function:histone lysine N-methyltransferase activity (H3-K4 specific); protein binding Biological Process: histone H3-K4 methylation
NCBI Summary:	TMEM113 (WDR82) is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes (Lee and Skalnik, 2005 [PubMed 16253997]; Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Jul 2010]
UniProt Code:	Q6UXN9
NCBI GenInfo Identifier:	74758580
NCBI Gene ID:	80335
NCBI Accession:	Q6UXN9.1
UniProt Secondary Accession:	Q6UXN9,Q8TEB2, A8K5R5,
UniProt Related Accession:	Q6UXN9
Molecular Weight:	35,079 Da
NCBI Full Name:	WD repeat-containing protein 82
NCBI Synonym Full Names:	WD repeat domain 82
NCBI Official Symbol:	WDR82
NCBI Official Synonym Symbols:	SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047
NCBI Protein Information:	WD repeat-containing protein 82
UniProt Protein Name:	WD repeat-containing protein 82
UniProt Synonym Protein Names:	Protein TMEM113; Swd2
Protein Family:	WD repeat-containing protein
UniProt Gene Name:	WDR82
UniProt Entry Name:	WDR82_HUMAN

Western blot analysis of extracts of various cell lines, using WDR82 antibody (CAB15518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)