Anti-WASF1 Antibody (CAB14624)
- SKU:
- CAB14624
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-WASF1 Antibody |
| Antibody SKU: | CAB14624 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human WASF1 (NP_001020107.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG, DU145, HeLa, K-562, 293T |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human WASF1 (NP_001020107.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VARA APVC ETVP VHPL PQGE VQGL PPPP PPPP LPPP GIRP SSPV TVTA LAHP PSGL HPTP STAP GPHV PLMP PSPP SQVI PASE PKRH PSTL PVIS DARS VLLE AIRK GIQL RKVE EQRE QEAK HERI ENDV ATIL SRRI AVEY SDSE DDSE FDEV DWLE |
| Gene ID: | 8936 |
| Uniprot: | Q92558 |
| Cellular Location: | Cell junction, Cytoplasm, cytoskeleton, focal adhesion, synapse |
| Calculated MW: | 61kDa |
| Observed MW: | 62kDa |
| Synonyms: | WASF1, SCAR1, WAVE, WAVE1 |
| Background: | The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | WAVE1: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Belongs to the SCAR/WAVE family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 6q21 Cellular Component: actin cytoskeleton; cytoskeleton; focal adhesion; lamellipodium; mitochondrial outer membrane; synapse Molecular Function:actin binding; protein binding; protein complex binding; Rac GTPase binding Biological Process: actin filament polymerization; cell motility; protein complex assembly; Rac protein signal transduction |
| NCBI Summary: | The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92558 |
| NCBI GenInfo Identifier: | 2495730 |
| NCBI Gene ID: | 8936 |
| NCBI Accession: | Q92558.1 |
| UniProt Secondary Accession: | Q92558,Q5SZK7, E1P5F2, |
| UniProt Related Accession: | Q92558 |
| Molecular Weight: | 61,652 Da |
| NCBI Full Name: | Wiskott-Aldrich syndrome protein family member 1 |
| NCBI Synonym Full Names: | WAS protein family member 1 |
| NCBI Official Symbol: | WASF1 |
| NCBI Official Synonym Symbols: | WAVE; SCAR1; WAVE1 |
| NCBI Protein Information: | wiskott-Aldrich syndrome protein family member 1 |
| UniProt Protein Name: | Wiskott-Aldrich syndrome protein family member 1 |
| UniProt Synonym Protein Names: | Protein WAVE-1; Verprolin homology domain-containing protein 1 |
| Protein Family: | Wiskott-Aldrich syndrome protein family |
| UniProt Gene Name: | WASF1 |
| UniProt Entry Name: | WASF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WASF1 antibody (CAB14624) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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