Anti-VPS54 Antibody (CAB13215)
- SKU:
- CAB13215
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-VPS54 Antibody |
| Antibody SKU: | CAB13215 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 420-720 of human VPS54 (NP_001005739.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, Mouse kidney, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 420-720 of human VPS54 (NP_001005739.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VVKL ADQM RMLN FPQW FDLL KDIF SKFT IFLQ RVKA TLNI IHSV VLSV LDKN QRTR ELEE ISQQ KNAA KDNS LDTE VAYL IHEG MFIS DAFG EGEL TPIA VDTT SQRN ASPN SEPC SSDS VSEP ECTT DSSS SKEH TSSS AIPG GVDI MVSE DMKL TDSE LGKL ANNI QELL YSAS DICH DRAV KFLM SRAK DGFL EKLN SMEF ITLS RLME TFIL DTEQ ICGR KSTS LLGA LQSQ AIKF VNRF HEER KTKL SLLL DNER WKQA DVPA EFQD LVDS LSDG KIAL PEKK SGAT EERK PAEV L |
| Gene ID: | 51542 |
| Uniprot: | Q9P1Q0 |
| Cellular Location: | Golgi apparatus, trans-Golgi network |
| Calculated MW: | 15kDa/93kDa/109kDa/110kDa |
| Observed MW: | 110kDa |
| Synonyms: | VPS54, HCC8, PPP1R164, SLP-8p, VPS54L, WR, hVps54L |
| Background: | This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms |
| UniProt Protein Function: | Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061). |
| NCBI Summary: | This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9P1Q0 |
| NCBI GenInfo Identifier: | 82583721 |
| NCBI Gene ID: | 51542 |
| NCBI Accession: | Q9P1Q0.2 |
| UniProt Secondary Accession: | Q9P1Q0,Q5VIR5, Q86YF7, Q8N6G3, Q9NPV0, Q9NT07, Q9NUJ0 |
| UniProt Related Accession: | Q9P1Q0 |
| Molecular Weight: | 15,982 Da |
| NCBI Full Name: | Vacuolar protein sorting-associated protein 54 |
| NCBI Synonym Full Names: | VPS54, GARP complex subunit |
| NCBI Official Symbol: | VPS54 |
| NCBI Official Synonym Symbols: | WR; HCC8; SLP-8p; VPS54L; hVps54L; PPP1R164 |
| NCBI Protein Information: | vacuolar protein sorting-associated protein 54 |
| UniProt Protein Name: | Vacuolar protein sorting-associated protein 54 |
| UniProt Synonym Protein Names: | Hepatocellular carcinoma protein 8; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8p |
| UniProt Gene Name: | VPS54 |
| UniProt Entry Name: | VPS54_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VPS54 antibody (CAB13215) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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