Anti-UBE2Z Antibody (CAB7225)
- SKU:
- CAB7225
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-UBE2Z Antibody |
| Antibody SKU: | CAB7225 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 115-354 of human UBE2Z (NP_075567.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, MCF7, HepG2, HeLa, Mouse pancreas, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 115-354 of human UBE2Z (NP_075567.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPPG MFVV PDTV DMTK IHAL ITGP FDTP YEGG FFLF VFRC PPDY PIHP PRVK LMTT GNNT VRFN PNFY RNGK VCLS ILGT WTGP AWSP AQSI SSVL ISIQ SLMT ENPY HNEP GFEQ ERHP GDSK NYNE CIRH ETIR VAVC DMME GKCP CPEP LRGV MEKS FLEY YDFY EVAC KDRL HLQG QTMQ DPFG EKRG HFDY QSLL MRLG LIRQ KVLE RLHN ENAE MDSD SSSS GTET DLHG SLRV |
| Gene ID: | 65264 |
| Uniprot: | Q9H832 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 28kDa/38kDa |
| Observed MW: | 25kDa/38kDa |
| Synonyms: | UBE2Z, HOYS7, USE1 |
| Background: | This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis. |
| UniProt Protein Function: | UBE2Z: Catalyzes the covalent attachment of ubiquitin to other proteins. Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: cytoplasm; nucleoplasm Molecular Function:ATP binding; protein binding; ubiquitin protein ligase binding Biological Process: apoptosis; protein ubiquitination during ubiquitin-dependent protein catabolic process |
| NCBI Summary: | This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q9H832 |
| NCBI GenInfo Identifier: | 134035344 |
| NCBI Gene ID: | 65264 |
| NCBI Accession: | Q9H832.2 |
| UniProt Secondary Accession: | Q9H832,Q7L354, Q8TCM4, Q9H893, A6N8M6, A6NC60, |
| UniProt Related Accession: | Q9H832 |
| Molecular Weight: | 28,075 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 Z |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 Z |
| NCBI Official Symbol: | UBE2Z |
| NCBI Official Synonym Symbols: | USE1; HOYS7 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 Z |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 Z |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme Z; Uba6-specific E2 conjugating enzyme 1; Use1; Ubiquitin carrier protein Z; Ubiquitin-protein ligase Z |
| Protein Family: | Protein transport protein |
| UniProt Gene Name: | UBE2Z |
| UniProt Entry Name: | UBE2Z_HUMAN |
 | Western blot analysis of extracts of various cell lines, using UBE2Z antibody (CAB7225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human kidney using UBE2Z antibody (CAB7225) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using UBE2Z antibody (CAB7225) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using UBE2Z antibody (CAB7225). Blue: DAPI for nuclear staining. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
