Anti-TXNRD2 Antibody (CAB5490)
- SKU:
- CAB5490
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TXNRD2 Antibody |
| Antibody SKU: | CAB5490 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human TXNRD2 (NP_006431.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, HepG2, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human TXNRD2 (NP_006431.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AALL GGLI QDAP NYGW EVAQ PVPH DWRK MAEA VQNH VKSL NWGH RVQL QDRK VKYF NIKA SFVD EHTV CGVA KGGK EILL SADH IIIA TGGR PRYP THIE GALE YGIT SDDI FWLK ESPG KTLV VGAS YVAL ECAG FLTG IGLD TTIM MRSI PLRG FDQQ MSSM VIEH MASH GTRF LRGC APSR VRRL PDGQ LQVT WEDS TTGK EDTG TFD |
| Gene ID: | 10587 |
| Uniprot: | Q9NNW7 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 30kDa/46kDa/53kDa/56kDa |
| Observed MW: | 56kDa |
| Synonyms: | TXNRD2, SELZ, TR, TR-BETA, TR3, TRXR2 |
| Background: | The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. |
| UniProt Protein Function: | TXNRD2: Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox- regulated cell signaling. Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; EC 1.8.1.9; Oxidoreductase Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:FAD binding; protein binding; thioredoxin-disulfide reductase activity Biological Process: cell redox homeostasis; response to oxygen radical; response to reactive oxygen species |
| NCBI Summary: | This gene encodes a member of the class I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein is a selenocysteine-containing flavoenzyme that maintains thioredoxins in a reduced state, thereby playing a key role in regulating the cellular redox environment. Mammals have three related thioredoxin reductases. This gene encodes a mitochondrial form important for scavenging of reactive oxygen species in mitochondria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2013] |
| UniProt Code: | Q9NNW7 |
| NCBI GenInfo Identifier: | 182705230 |
| NCBI Gene ID: | 10587 |
| NCBI Accession: | Q9NNW7.3 |
| UniProt Secondary Accession: | Q9NNW7,O95840, Q96IJ2, Q9H2Z5, Q9NZV3, Q9NZV4, Q9P2Y0 Q9P2Y1, Q9UQU8, |
| UniProt Related Accession: | Q9NNW7 |
| Molecular Weight: | 30,291 Da |
| NCBI Full Name: | Thioredoxin reductase 2, mitochondrial |
| NCBI Synonym Full Names: | thioredoxin reductase 2 |
| NCBI Official Symbol: | TXNRD2 |
| NCBI Official Synonym Symbols: | TR; TR3; SELZ; TRXR2; TR-BETA |
| NCBI Protein Information: | thioredoxin reductase 2, mitochondrial |
| UniProt Protein Name: | Thioredoxin reductase 2, mitochondrial |
| UniProt Synonym Protein Names: | Selenoprotein Z; SelZ; TR-beta; Thioredoxin reductase TR3 |
| Protein Family: | Thioredoxin reductase |
| UniProt Gene Name: | TXNRD2 |
| UniProt Entry Name: | TRXR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TXNRD2 antibody (CAB5490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using TXNRD2 antibody (CAB5490) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using TXNRD2 antibody (CAB5490) at dilution of 1:100 (40x lens). |
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