Anti-TRIM59 Antibody (CAB13876)
- SKU:
- CAB13876
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TRIM59 Antibody |
| Antibody SKU: | CAB13876 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human TRIM59 (NP_775107.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, NIH/3T3, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human TRIM59 (NP_775107.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHNF EEEL TCPI CYSI FEDP RVLP CSHT FCRN CLEN ILQA SGNF YIWR PLRI PLKC PNCR SITE IAPT GIES LPVN FALR AIIE KYQQ EDHP DIVT CPEH YRQP LNVY CLLD KKLV CGHC LTIG QHHG HPID DLQS AYLK EKDT PQKL LEQL TDTH WTDL THLI EKLK EQKS HSEK MIQG DKEA VLQY FKEL NDTL EQKK KSFL TALC DVGN LINQ EYTP QIER MKEI REQQ LELM ALTI SLQE ESPL KFLE KVDD VRQH VQIL KQRP LPEV QPVE IYPR VSKI LKEE WSRT EIGQ IKNV LIPK MKIS PKRM SCSW PGKD EKEV EFLK IL |
| Gene ID: | 286827 |
| Uniprot: | Q8IWR1 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass membrane protein |
| Calculated MW: | 47kDa/123kDa |
| Observed MW: | 50kDa |
| Synonyms: | TRIM59, IFT80L, MRF1, RNF104, TRIM57, TSBF1 |
| Background: |
| UniProt Protein Function: | TRIM59: Belongs to the TRIM/RBCC family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Membrane protein, integral Chromosomal Location of Human Ortholog: 3q25.33 Cellular Component: centrosome; cilium; endoplasmic reticulum Molecular Function:protein binding Biological Process: cilium biogenesis; negative regulation of I-kappaB kinase/NF-kappaB cascade |
| UniProt Code: | Q8IWR1 |
| NCBI GenInfo Identifier: | 74714421 |
| NCBI Gene ID: | 286827 |
| NCBI Accession: | Q8IWR1.1 |
| UniProt Secondary Accession: | Q8IWR1,A8K5G9, D3DNL9, |
| UniProt Related Accession: | Q8IWR1 |
| Molecular Weight: | 123,321 Da |
| NCBI Full Name: | Tripartite motif-containing protein 59 |
| NCBI Synonym Full Names: | tripartite motif containing 59 |
| NCBI Official Symbol: | TRIM59 |
| NCBI Official Synonym Symbols: | MRF1; TSBF1; IFT80L; RNF104; TRIM57 |
| NCBI Protein Information: | tripartite motif-containing protein 59 |
| UniProt Protein Name: | Tripartite motif-containing protein 59 |
| UniProt Synonym Protein Names: | RING finger protein 104; Tumor suppressor TSBF-1 |
| Protein Family: | Tripartite motif-containing protein |
| UniProt Gene Name: | TRIM59 |
| UniProt Entry Name: | TRI59_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRIM59 antibody (CAB13876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using TRIM59 antibody (CAB13876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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