Anti-TMPO Antibody (CAB2534)[KO Validated]

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SKU:
CAB2534
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-TMPO Antibody [KO Validated]
Antibody SKU:CAB2534
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 293T, HepG2, mouse liver, rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPEF LEDP SVLT KDKL KSEL VANN VTLP AGEQ RKDV YVQL YLQH LTAR NRPP LPAG TNSK GPPD FSSD EERE PTPV LGSG AAAA GRSR AAVG RKAT KKTD KPRQ EDKD DLDV TELT NEDL LDQL VKYG VNPG PIVG TTRK LYEK KLLK LREQ GTES RSST PLPT ISSS AENT RQNG SNDS DRYS DNEE DSKI ELKL EKRE PLKG RAKT PVTL KQRR VEHN QSYS QAGI TETE WTSG SSKG GPLQ ALTR ESTR GSRR TPRK RVET SEHF RIDG PVIS ESTP IAET IMAS SNES LVVN RVTG NFKH ASPI LPIT EFSD IPRR APKK PLTR AEVG EKTE ERRV ERDI LKEM FPYE ASTP TGIS ASCR RPIK GAAG RPLE LSDF RMEE SFSS KYVP KYVP LADV KSEK TKKG RS
Gene ID:7112
Uniprot:P42166/P42167
Cellular Location:Chromosome, Nucleus
Calculated MW:38kDa/50kDa/75kDa
Observed MW:51kDa/80kDa
Synonyms:TMPO, CMD1T, LAP2, LEMD4, PRO0868, TP
Background:The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
UniProt Protein Function:TMPO iso6: a single-pass type II membrane protein. Tightly associated with the nuclear lamina. Binds DNA and involved in DNA replication and chromatin remodeling, May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with HAP95. Associated with dilated cardiomyopathy; upregulated in medulloblastoma. Used as a pharmaceutical to treat primary and secondary immune deficiencies, autoimmunity, infections and cancer. Available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Three alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:DNA-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: chromatin; nuclear envelope; nucleus

Molecular Function:lamin binding

Disease: Cardiomyopathy, Dilated, 1t

NCBI Summary:The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, May 2010]
UniProt Code:P42166
NCBI GenInfo Identifier:1174689
NCBI Gene ID:7112
NCBI Accession:P42166.2
UniProt Secondary Accession:P42166,P08918, P08919, Q14860, Q16295,
UniProt Related Accession:P42166,P42167
Molecular Weight:50,670 Da
NCBI Full Name:Lamina-associated polypeptide 2, isoform alpha
NCBI Synonym Full Names:thymopoietin
NCBI Official Symbol:TMPO  
NCBI Official Synonym Symbols:TP; LAP2; CMD1T; LEMD4; PRO0868  
NCBI Protein Information:thymopoietin
UniProt Protein Name:Lamina-associated polypeptide 2, isoform alpha
UniProt Synonym Protein Names:Thymopoietin isoform alpha; TP alpha
Protein Family:Lamina-associated polypeptide
UniProt Gene Name:TMPO  
UniProt Entry Name:LAP2A_HUMAN
Anti-TMPO Antibody [KO Validated] (CAB2534)Western blot analysis of extracts of various cell lines, using TMPO antibody (CAB2534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TMPO Antibody [KO Validated] (CAB2534)Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (CAB2534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-TMPO Antibody [KO Validated] (CAB2534)Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).
Anti-TMPO Antibody [KO Validated] (CAB2534)Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).
Anti-TMPO Antibody [KO Validated] (CAB2534)Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).

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