Anti-SUCLG2 Antibody (CAB8976)
- SKU:
- CAB8976
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-SUCLG2 Antibody |
| Antibody SKU: | CAB8976 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, 293T, LO2, U-87MG, MCF7, Raji, Mouse spleen, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SDNG VRVQ RFFV ADTA NEAL EAAK RLNA KEIV LKAQ ILAG GRGK GVFN SGLK GGVH LTKD PNVV GQLA KQMI GYNL ATKQ TPKE GVKV NKVM VAEA LDIS RETY LAIL MDRS CNGP VLVG SPQG GVDI EEVA ASNP ELIF KEQI DIFE GIKD SQAQ RMAE NLGF VGPL KSQA ADQI TKLY NLFL KIDA TQVE VNPF GETP EGQV VCFD AKIN FDDN AEFR QKDI FAMD DKSE NEPI ENEA AKYD LKYI GLD |
| Gene ID: | 8801 |
| Uniprot: | Q96I99 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 46kDa/47kDa |
| Observed MW: | 47kDa |
| Synonyms: | SUCLG2, G-SCS, GBETA, GTPSCS |
| Background: | This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12. |
| UniProt Protein Function: | SUCLG2: Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Belongs to the succinate/malate CoA ligase beta subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - propanoate; EC 6.2.1.4; Ligase; Mitochondrial Chromosomal Location of Human Ortholog: 3p14.1 Cellular Component: mitochondrial matrix; mitochondrion; plasma membrane Biological Process: tricarboxylic acid cycle |
| NCBI Summary: | This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12. [provided by RefSeq, Apr 2010] |
| UniProt Code: | Q96I99 |
| NCBI GenInfo Identifier: | 294862256 |
| NCBI Gene ID: | 8801 |
| NCBI Accession: | NP_001171070.1 |
| UniProt Secondary Accession: | Q96I99,O95195, Q6NUH7, Q86VX8, Q8WUQ1, C9JVT2, |
| UniProt Related Accession: | Q96I99 |
| Molecular Weight: | 56.3kD |
| NCBI Full Name: | succinate--CoA ligase |
| NCBI Synonym Full Names: | succinate-CoA ligase GDP-forming beta subunit |
| NCBI Official Symbol: | SUCLG2 |
| NCBI Official Synonym Symbols: | G-SCS; GBETA; GTPSCS |
| NCBI Protein Information: | succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial; succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial |
| UniProt Protein Name: | Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial |
| UniProt Synonym Protein Names: | GTP-specific succinyl-CoA synthetase subunit beta |
| UniProt Gene Name: | SUCLG2 |
 | Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (CAB8976) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using SUCLG2 antibody (CAB8976) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using SUCLG2 antibody (CAB8976) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using Anti-SUCLG2 Polyclonal Antibody (CAB8976) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Anti-SUCLG2 Polyclonal Antibody (CAB8976) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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