Anti-SFTPA1 항체 (CAB3133)

Antibody Name:	Anti-SFTPA1 Antibody
Antibody SKU:	CAB3133
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human SFTPA1

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Rat lung

Immunogen:	A synthetic peptide of human SFTPA1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	653509
Uniprot:	Q8IWL2
Cellular Location:	Secreted, extracellular matrix, extracellular space, surface film
Calculated MW:	26kDa/27kDa
Observed MW:	30KDa

Synonyms:

SFTPA1, COLEC4, PSAP, PSP-A, PSPA, SFTP1, SFTPA1B, SP-A, SP-A1, SPA, SPA1

Background:

This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	SFTPA1B: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Genetic variations in SFTPA1 are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Genetic variations in SFTPA1 are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. The association between SFTPA1 alleles and respiratory distress syndrome in premature infants is dependent on a variation Ile to Thr at position 131 in SFTPB. Belongs to the SFTPA family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 10q22.3 Cellular Component: extracellular space; proteinaceous extracellular matrix; collagen; extracellular region Molecular Function:lipid transporter activity; carbohydrate binding Biological Process: respiratory gaseous exchange; lipid transport Disease: Pulmonary Fibrosis, Idiopathic
NCBI Summary:	This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
UniProt Code:	Q8IWL2
NCBI GenInfo Identifier:	60416440
NCBI Gene ID:	653509
NCBI Accession:	Q8IWL2.2
UniProt Secondary Accession:	Q8IWL2,P07714, Q14DV4, Q5RIR5, Q5RIR7, A8K3T8, B7ZW50 E3VLD8, E3VLD9, E3VLE0, E3VLE1, G5E9J3,
UniProt Related Accession:	Q8IWL2
Molecular Weight:	27,736 Da
NCBI Full Name:	Pulmonary surfactant-associated protein A1
NCBI Synonym Full Names:	surfactant protein A1
NCBI Official Symbol:	SFTPA1
NCBI Official Synonym Symbols:	SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B
NCBI Protein Information:	pulmonary surfactant-associated protein A1; collectin-4; surfactant protein A1B; alveolar proteinosis protein; surfactant protein A1 variant AD' 6A; surfactant protein A1 variant ACD' 6A; surfactant protein A1 variant AD' 6A2; surfactant protein A1 variant AD' 6A3; surfactant protein A1 variant AD' 6A4; surfactant protein A1 variant AB'D' 6A; surfactant protein A1 variant ACD' 6A2; surfactant protein A1 variant ACD' 6A3; surfactant protein A1 variant ACD' 6A4; surfactant protein A1 variant AB'D' 6A2; surfactant protein A1 variant AB'D' 6A3; surfactant protein A1 variant AB'D' 6A4; surfactant, pulmonary-associated protein A1A; surfactant, pulmonary-associated protein A1B; 35 kDa pulmonary surfactant-associated protein
UniProt Protein Name:	Pulmonary surfactant-associated protein A1
UniProt Synonym Protein Names:	35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4
Protein Family:	Pulmonary surfactant-associated protein
UniProt Gene Name:	SFTPA1
UniProt Entry Name:	SFTA1_HUMAN

	Western blot analysis of extracts of various cell lines, using SFTPA1 antibody (CAB3133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat lung using SFTPA1 Rabbit pAb (CAB3133) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SFTPA1 Rabbit pAb (CAB3133) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using SFTPA1 Rabbit pAb (CAB3133) at dilution of 1:50 (40x lens).

Antibodies	ELISA Kits
SFTPA1 Antibody (PACO12154)	Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit (HUES02324)
	Human Pulmonary surfactant-associated protein A1 (SFTPA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)