Anti-SFPQ Antibody (CAB0958)
- SKU:
- CAB0958
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SFPQ Antibody |
| Antibody SKU: | CAB0958 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 578-707 of human SFPQ (NP_005057.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | OVCAR3, HeLa, Jurkat, HepG2, Mouse brain, Mouse liver, Mouse spleen, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 578-707 of human SFPQ (NP_005057.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MMIR QREM EEQM RRQR EESY SRMG YMDP RERD MRMG GGGA MNMG DPYG SGGQ KFPP LGGG GGIG YEAN PGVP PATM SGSM MGSD MRTE RFGQ GGAG PVGG QGPR GMGP GTPA GYGR GREE YEGP NKKP RF |
| Gene ID: | 6421 |
| Uniprot: | P23246 |
| Cellular Location: | Cytoplasm, Nucleus matrix |
| Calculated MW: | 72kDa/76kDa |
| Observed MW: | 115kDa |
| Synonyms: | SFPQ, POMP100, PPP1R140, PSF |
| Background: | DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA . |
| UniProt Protein Function: | PSF: a proline-and glutamine-rich splicing factor. Essential pre-mRNA splicing factor required early in spliceosome formation. Seems to also bind DNA. The active complex is a heterotetramer of two 52 kDa and two 100 kDa subunits. Reported to be a PKC-alpha-binding protein in the cell nucleus. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus; paraspeckles; chromatin Molecular Function:protein binding; histone deacetylase binding; nucleotide binding Biological Process: alternative nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; DNA repair; mRNA processing; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; DNA recombination |
| UniProt Code: | P23246 |
| NCBI GenInfo Identifier: | 1709851 |
| NCBI Gene ID: | 6421 |
| NCBI Accession: | P23246.2 |
| UniProt Secondary Accession: | P23246,P30808, Q5SZ71, |
| UniProt Related Accession: | P23246 |
| Molecular Weight: | Calculated MW: 72kDa/76kDaObserved MW: 115kDa |
| NCBI Full Name: | Splicing factor, proline- and glutamine-rich |
| NCBI Synonym Full Names: | splicing factor proline/glutamine-rich |
| NCBI Official Symbol: | SFPQ |
| NCBI Official Synonym Symbols: | PSF; POMP100; PPP1R140 |
| NCBI Protein Information: | splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract-binding protein-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated) |
| UniProt Protein Name: | Splicing factor, proline- and glutamine-rich |
| UniProt Synonym Protein Names: | 100 kDa DNA-pairing protein; hPOMp100; DNA-binding p52/p100 complex, 100 kDa subunit; Polypyrimidine tract-binding protein-associated-splicing factor; PSF; PTB-associated-splicing factor |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | SFPQ |
| UniProt Entry Name: | SFPQ_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SFPQ antibody (CAB0958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human esophageal using SFPQ Rabbit pAb (CAB0958) at dilution of 1:25 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using SFPQ Rabbit pAb (CAB0958) at dilution of 1:25 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SFPQ antibody (CAB0958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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