Anti-SERPINB1 Antibody (CAB6257)
- SKU:
- CAB6257
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SERPINB1 Antibody |
| Antibody SKU: | CAB6257 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human SERPINB1 (NP_109591.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HeLa, MCF7, A-549, 22Rv1, Mouse lung, Mouse lung, Mouse spleen, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human SERPINB1 (NP_109591.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FLPE FLVS TQKT YGAD LASV DFQH ASED ARKT INQW VKGQ TEGK IPEL LASG MVDN MTKL VLVN AIYF KGNW KDKF MKEA TTNA PFRL NKKD RKTV KMMY QKKK FAYG YIED LKCR VLEL PYQG EELS MVIL LPDD IEDE STGL KKIE EQLT LEKL HEWT KPEN LDFI EVNV SLPR FKLE ESYT LNSD LARL GVQD LFNS SKAD LSGM SGAR DIFI SKIV HKSF VEVN EEGT EAAA ATAG IATF CMLM PEEN FTAD HPFL FFIR HNSS GSIL FLGR FSSP |
| Gene ID: | 1992 |
| Uniprot: | P30740 |
| Cellular Location: | Cytoplasm, Cytoplasmic granule |
| Calculated MW: | 26kDa/42kDa |
| Observed MW: | 43kDa |
| Synonyms: | SERPINB1, EI, ELANH2, HEL-S-27, HEL57, LEI, M/NEI, MNEI, PI-2, PI2 |
| Background: | The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 6p25 Cellular Component: extracellular space; membrane; cytoplasm Molecular Function:serine-type endopeptidase inhibitor activity |
| NCBI Summary: | The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| UniProt Code: | P30740 |
| NCBI GenInfo Identifier: | 266344 |
| NCBI Gene ID: | 1992 |
| NCBI Accession: | P30740.1 |
| UniProt Secondary Accession: | P30740,Q53FB9, Q5W0E1, Q9UDF8, A8K5L2, B4DNT0, |
| UniProt Related Accession: | P30740 |
| Molecular Weight: | Calculated MW: 26kDa/42kDaObserved: 43kDa |
| NCBI Full Name: | Leukocyte elastase inhibitor |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade B (ovalbumin), member 1 |
| NCBI Official Symbol: | SERPINB1 |
| NCBI Official Synonym Symbols: | EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2 |
| NCBI Protein Information: | leukocyte elastase inhibitor; peptidase inhibitor 2; epididymis luminal protein 57; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1 |
| UniProt Protein Name: | Leukocyte elastase inhibitor |
| UniProt Synonym Protein Names: | Monocyte/neutrophil elastase inhibitor; EI; M/NEI; Peptidase inhibitor 2; PI-2; Serpin B1 |
| Protein Family: | Leukocyte elastase inhibitor |
| UniProt Gene Name: | SERPINB1 |
| UniProt Entry Name: | ILEU_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SERPINB1 antibody (CAB6257) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 60s. |
 | Immunofluorescence analysis of HeLa cells using SERPINB1 antibody (CAB6257). Blue: DAPI for nuclear staining. |
Related Products
Anti-SERPINB13 Antibody (CAB13744)
