Anti-SEC61B Antibody (CAB15788)[KO Validated]

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SKU:
CAB15788
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-SEC61B Antibody [KO Validated]
Antibody SKU:CAB15788
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, HepG2, SH-SY5Y, mouse brain, mouse stomach
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPGP TPSG TNVG SSGR SPSK AVAA RAAG STVR QRKN ASCG TRSA GRTT SAGT GGMW RFYT EDSP GLKV GPVP VLVM SLLF IASV FMLH IWGK YTRS
Gene ID:10952
Uniprot:P60468
Cellular Location:Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:9kDa
Observed MW:12kDa
Synonyms:SEC61B
Background:The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
UniProt Protein Function:SEC61B: Necessary for protein translocation in the endoplasmic reticulum. Belongs to the SEC61-beta family.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.33

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Molecular Function:protein binding; epidermal growth factor binding

Biological Process: retrograde protein transport, ER to cytosol; SRP-dependent cotranslational protein targeting to membrane; antigen processing and presentation of peptide antigen via MHC class I; ER-associated protein catabolic process; cellular protein metabolic process; translation; protein import into nucleus, translocation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression

NCBI Summary:The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008]
UniProt Code:P60468
NCBI GenInfo Identifier:42560366
NCBI Gene ID:10952
NCBI Accession:P60468.2
UniProt Related Accession:P60468
Molecular Weight:
NCBI Full Name:Protein transport protein Sec61 subunit beta
NCBI Synonym Full Names:SEC61 translocon beta subunit
NCBI Official Symbol:SEC61B  
NCBI Protein Information:protein transport protein Sec61 subunit beta
UniProt Protein Name:Protein transport protein Sec61 subunit beta
UniProt Gene Name:SEC61B  
UniProt Entry Name:SC61B_HUMAN
Anti-SEC61B Antibody [KO Validated] (CAB15788)Western blot analysis of extracts of various cell lines, using SEC61B antibody (CAB15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s.
Anti-SEC61B Antibody [KO Validated] (CAB15788)Western blot analysis of extracts from normal (control) and SEC61B knockout (KO) 293T cells, using SEC61B antibody (CAB15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-SEC61B Antibody [KO Validated] (CAB15788)Immunofluorescence analysis of L929 cells using SEC61B antibody (CAB15788) at dilution of 1:100. Blue: DAPI for nuclear staining.

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