Anti-RRAGD Antibody (CAB9979)
- SKU:
- CAB9979
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-RRAGD Antibody |
| Antibody SKU: | CAB9979 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 181-400 of human RRAGD (NP_067067.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:1000 - 1:4000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, NCI-H460, SW480, HL-60, 22Rv1, Mouse heart, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 181-400 of human RRAGD (NP_067067.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VDGL SDDH KIET QRDI HQRA NDDL ADAG LEKI HLSF YLTS IYDH SIFE AFSK VVQK LIPQ LPTL ENLL NIFI SNSG IEKA FLFD VVSK IYIA TDST PVDM QTYE LCCD MIDV VIDI SCIY GLKE DGAG TPYD KEST AIIK LNNT TVLY LKEV TKFL ALVC FVRE ESFE RKGL IDYN FHCF RKAI HEVF EVRM KVVK SRKV QNRL QKKK RATP NGTP RVLL |
| Gene ID: | 58528 |
| Uniprot: | Q9NQL2 |
| Cellular Location: | Cytoplasm, Lysosome, Nucleus |
| Calculated MW: | 28kDa/45kDa |
| Observed MW: | 46kDa |
| Synonyms: | RRAGD, RAGD, bA11D8.2.1 |
| Background: |
| UniProt Protein Function: | RRAGD: Has guanine nucleotide-binding activity but lacks intrinsic GTPase activity. Probably required for the amino acid- induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Forms a heterodimer with RRAGA in a sequence-independent manner. Binds GTP. Interacts with NOL8 and RRAGB. Belongs to the GTR/RAG GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein regulator, misc. Chromosomal Location of Human Ortholog: 6q15 Cellular Component: cytoplasm; cytosol; lysosome; nucleus Molecular Function:GTPase activity; protein binding; protein heterodimerization activity Biological Process: cell cycle arrest; cellular response to starvation; macroautophagy; positive regulation of TOR signaling pathway; regulation of autophagy |
| NCBI Summary: | RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.[supplied by OMIM, Apr 2004] |
| UniProt Code: | Q9NQL2 |
| NCBI GenInfo Identifier: | 74752904 |
| NCBI Gene ID: | 58528 |
| NCBI Accession: | Q9NQL2.1 |
| UniProt Secondary Accession: | Q9NQL2,Q7L8F9, Q9NPG0, A8K184, |
| UniProt Related Accession: | Q9NQL2 |
| Molecular Weight: | 28,611 Da |
| NCBI Full Name: | Ras-related GTP-binding protein D |
| NCBI Synonym Full Names: | Ras related GTP binding D |
| NCBI Official Symbol: | RRAGD |
| NCBI Official Synonym Symbols: | RAGD; bA11D8.2.1 |
| NCBI Protein Information: | ras-related GTP-binding protein D |
| UniProt Protein Name: | Ras-related GTP-binding protein D |
| UniProt Gene Name: | RRAGD |
| UniProt Entry Name: | RRAGD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RRAGD antibody (CAB9979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using RRAGD antibody (CAB9979) at dilution of 1:100 (40x lens). |
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